Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) Standard Curve.)
Rat Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit | MAP1LC3B elisa kit
Rat Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit
Gene Names
Map1lc3b; LC3B; Mpl3; zbs559; Map1lc3
Reactivity
Rat
Synonyms
Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B); Rat Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit; MAP1LC3B; LC3B; MAP1A/1BLC3; microtubule-associated proteins 1A/1B light chain 3; MAP1LC3B elisa kit
Reactivity
Rat
Specificity
Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAP1LC3B. No significant cross-reactivity or interference between Rat MAP1LC3B and analogues was observed.
Sequence Length
125
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) Standard Curve.)
Related Product Information for MAP1LC3B elisa kit
Description: This Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3B in samples. An antibody specific for MAP1LC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP1LC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification. The essential site for modification of MAP1LC3B was lys122.
Background: MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification. The essential site for modification of MAP1LC3B was lys122.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3B
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol
Map1lc3b
NCBI Official Synonym Symbols
LC3B; Mpl3; zbs559; Map1lc3
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3B
Protein Family
UniProt Gene Name
Map1lc3b
UniProt Synonym Gene Names
Map1alc3; Map1lc3; MAP1A/MAP1B LC3 B
UniProt Entry Name
MLP3B_RAT
NCBI Description
may mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system [RGD, Feb 2006]
Research Articles on MAP1LC3B
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Product Notes
The Rat MAP1LC3B map1lc3b (Catalog #AAA9712334) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712334 ELISA Kit recognizes Rat MAP1LC3B. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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