Typical Testing Data/Standard Curve (for reference only)
Rat Microtubule-associated Proteins 1A,1B Light Chain 3B ELISA Kit | MAP1LC3B elisa kit
Rat Microtubule-associated Proteins 1A,1B Light Chain 3B ELISA Kit
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Rat
Synonyms
Microtubule-associated Proteins 1A; 1B Light Chain 3B; Rat Microtubule-associated Proteins 1A; 1B Light Chain 3B ELISA Kit; MAP1LC3B elisa kit
Reactivity
Rat
Sequence Length
125
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.3ng/ml-90ng/ml
Sensitivity
0.16ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MAP1LC3B elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Microtubule-associated Protein Light Chain 3 (also known as MAP1LC3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MAP1LC3 antibody. MAP1LC3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MAP1LC3 Antibody is added and binds to MAP1LC3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MAP1LC3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MAP1LC3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MAP1LC3 antibody. MAP1LC3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MAP1LC3 Antibody is added and binds to MAP1LC3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MAP1LC3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MAP1LC3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3B
NCBI Official Synonym Full Names
microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol
MAP1LC3B
NCBI Official Synonym Symbols
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3B
Protein Family
UniProt Gene Name
MAP1LC3B
UniProt Synonym Gene Names
MAP1ALC3; MAP1A/MAP1B LC3 B
UniProt Entry Name
MLP3B_HUMAN
NCBI Description
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
Research Articles on MAP1LC3B
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Product Notes
The Rat MAP1LC3B map1lc3b (Catalog #AAA1603471) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603471 ELISA Kit recognizes Rat MAP1LC3B. It is sometimes possible for the material contained within the vial of "Microtubule-associated Proteins 1A,1B Light Chain 3B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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