Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Spermatid nuclear transition protein 1 (TNP1) Standard Curve.)
Rat Spermatid Nuclear Transition Protein 1 (TNP1) ELISA Kit | TNP1 elisa kit
Rat Spermatid Nuclear Transition Protein 1 (TNP1) ELISA Kit
Reactivity
Rat
Synonyms
Spermatid Nuclear Transition Protein 1 (TNP1); Rat Spermatid Nuclear Transition Protein 1 (TNP1) ELISA Kit; TNP1; TP1; TNP1 elisa kit
Reactivity
Rat
Specificity
Rat Spermatid nuclear transition protein 1 (TNP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNP1. No significant cross-reactivity or interference between Rat TNP1 and analogues was observed.
Sequence Length
55
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Spermatid nuclear transition protein 1 (TNP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Spermatid nuclear transition protein 1 (TNP1) Standard Curve.)
Related Product Information for TNP1 elisa kit
Description: This Rat Spermatid nuclear transition protein 1 (TNP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNP1 in samples. An antibody specific for TNP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Yu et al. (2000) generated mice lacking the major transition nuclear protein, TP1, by targeted deletion of the Tnp1 gene in mouse embryonic stem cells. Surprisingly, testis weights and sperm production were normal in the mutant mice, and only subtle abnormalities were detected in sperm morphology. Electron microscopy showed large rod-like structures in the chromatin of mutant step 13 spermatids, in contrast to the fine chromatin fibrils observed in wildtype. Steps 12-13 spermatid nuclei from the testis of Tnp1-null mice contained, in place of TP1, elevated levels of TP2 (TNP2) and some PRM2 precursor.
Background: Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Yu et al. (2000) generated mice lacking the major transition nuclear protein, TP1, by targeted deletion of the Tnp1 gene in mouse embryonic stem cells. Surprisingly, testis weights and sperm production were normal in the mutant mice, and only subtle abnormalities were detected in sperm morphology. Electron microscopy showed large rod-like structures in the chromatin of mutant step 13 spermatids, in contrast to the fine chromatin fibrils observed in wildtype. Steps 12-13 spermatid nuclei from the testis of Tnp1-null mice contained, in place of TP1, elevated levels of TP2 (TNP2) and some PRM2 precursor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
spermatid nuclear transition protein 1
NCBI Official Synonym Full Names
transition protein 1
NCBI Official Symbol
Tnp1
NCBI Protein Information
spermatid nuclear transition protein 1
UniProt Protein Name
Spermatid nuclear transition protein 1
Protein Family
UniProt Gene Name
Tnp1
UniProt Synonym Gene Names
STP-1; TP-1
UniProt Entry Name
STP1_RAT
NCBI Description
spermatid transitional protein; highly basic protein expressed during the brief period when histones are being replaced by protamines in the haploid stage of spermatogenesis [RGD, Feb 2006]
Uniprot Description
TP1: In the elongating spermatids of mammals, the conversion of nucleosomal chromatin to the compact, non-nucleosomal form found in the sperm nucleus is associated with the appearance of a small set of basic chromosomal transition proteins. Belongs to the nuclear transition protein 1 family.
Protein type: DNA-binding
Cellular Component: male germ cell nucleus; nucleosome
Molecular Function: DNA binding
Biological Process: chromatin remodeling; single strand break repair; sperm motility; spermatid nuclear elongation; nucleosome disassembly; sexual reproduction; multicellular organismal development; chromatin silencing; spermatogenesis; negative regulation of transcription, DNA-dependent; spermatid development
Research Articles on TNP1
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Product Notes
The Rat TNP1 tnp1 (Catalog #AAA9711657) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711657 ELISA Kit recognizes Rat TNP1. It is sometimes possible for the material contained within the vial of "Spermatid Nuclear Transition Protein 1 (TNP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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