Rabbit anti-Human, Mouse FAN1 Polyclonal Antibody | anti-FAN1 antibody
FAN1 Antibody
Gene Names
FAN1; KMIN; MTMR15; KIAA1018
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FAN1; Polyclonal Antibody; FAN1 Antibody; 6030441H18Rik; Coiled coil domain containing protein MTMR15; DKFZp451H236; DKFZp686K16147; FAN1_HUMAN; FANCD2 and FANCI associated nuclease 1; FANCD2/FANCI associated nuclease 1; FANCD2/FANCI-associated nuclease 1; Fanconi associated nuclease 1; Fanconi-associated nuclease 1; hFAN1; KIAA1018; KMIN; MTMR 15; MTMR15; Myotubularin related protein 15; Myotubularin-related protein 15; anti-FAN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
FAN1 Antibody detects endogenous levels of FAN1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-FAN1 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human FAN1.
Conjugation
Unconjugated
Similarity
The UBZ4-type zinc finger specifically binds monoubiquitinated FANCD2. The KEN box and D-box are required for interaction with FZR1/CDH1 and essential for APC(CDH1)-mediated ubiquitination. Belongs to the FAN1 family.
Post Translational Modifications
Ubiquitinated and degraded during mitotic exit by the APC/C-Cdh1 complex.
Subunit Structure
Interacts with FANCD2 (when monoubiquitinated). Interacts with FANCI, MLH1, MLH3 and PMS2.
Function
Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps.
Note
Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2 (PubMed:20603015, PubMed:20603016). Localizes to stalled replication forks via its UBZ4-type zinc finger (PubMed:20935496).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,225 Da
NCBI Official Full Name
fanconi-associated nuclease 1 isoform b
NCBI Official Synonym Full Names
FANCD2/FANCI-associated nuclease 1
NCBI Official Symbol
FAN1
NCBI Official Synonym Symbols
KMIN; MTMR15; KIAA1018
NCBI Protein Information
fanconi-associated nuclease 1; myotubularin-related protein 15; fanconi anemia associated nuclease 1; coiled-coil domain-containing protein MTMR15
UniProt Protein Name
Fanconi-associated nuclease 1
Protein Family
UniProt Gene Name
FAN1
UniProt Synonym Gene Names
KIAA1018; MTMR15
UniProt Entry Name
FAN1_HUMAN
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Product Notes
The FAN1 fan1 (Catalog #AAA9631356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAN1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the FAN1 fan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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