Rabbit RWDD3 Polyclonal Antibody | anti-RWDD3 antibody

RWDD3 Antibody

Cat. Num. AAA9631303

• Gene Names: RWDD3; RSUME
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RWDD3; Polyclonal Antibody; RWDD3 Antibody; DKFZp566K023; RSUME; RWD containing sumoylation enhancer; RWD domain containing 3; RWD domain containing protein; RWD domain containing protein 3; RWD domain-containing protein 3; RWD-containing sumoylation enhancer; rwdd3; RWDD3_HUMAN; anti-RWDD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RWDD3 Antibody detects endogenous levels of RWDD3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RWDD3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RWDD3.
• Conjugation: Unconjugated
• Similarity: The RWD domain is required for the sumoylation enhancement activity.
• Subunit Structure: Isoform 1 and isoform 2 interact with UBE2I/UBC9. Isoform 1 shows a greater interaction with NFKBIA and HIF1A as compared to isoform 2. Isoform 2 interacts with NCOA2 and NR3C1.
• Tissue Specificity: Isoform 1 and isoform 2 are expressed in glioma tumors (at protein level). Expressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal cord, esophagus, thymus, lung and peripheral blood leukocytes. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
• Function: Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination.
• Note: Colocalizes with UBC9/UBE2I in nuclear spots.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RWDD3 antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 153252154
• NCBI GeneID: 25950
• NCBI Accession #: NP_056300
• NCBI GenBank Nucleotide #: NM_015485.4
• UniProt Accession #: Q9Y3V2; Q08AJ7; Q6FID3; Q9BX35; A6NP44; A8K9F0; C9J9L7; C9JI45
• Molecular Weight: 22,857 Da
• NCBI Official Full Name: RWD domain-containing protein 3 isoform a
• NCBI Official Synonym Full Names: RWD domain containing 3
• NCBI Official Symbol: RWDD3
• NCBI Official Synonym Symbols: RSUME
• NCBI Protein Information: RWD domain-containing protein 3; RWD-containing sumoylation enhancer; RWD domain-containing sumoylation enhancer
• UniProt Protein Name: RWD domain-containing protein 3
• Protein Family: RWD domain-containing protein
• UniProt Gene Name: RWDD3
• UniProt Synonym Gene Names: RSUME; RSUME
• UniProt Entry Name: RWDD3_HUMAN

Uniprot Description

RWDD3: Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: SUMO conjugating system

Chromosomal Location of Human Ortholog: 1p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of protein sumoylation; inhibition of NF-kappaB transcription factor