Rabbit anti-Human, Mouse DEDD2 Polyclonal Antibody | anti-DEDD2 antibody

DEDD2 Antibody

Cat. Num. AAA9630876

• Gene Names: DEDD2; FLAME-3
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DEDD2; Polyclonal Antibody; DEDD2 Antibody; Death effector domain containing 2; Death effector domain-containing DNA-binding protein; Death effector domain-containing protein; Death effector domain-containing testicular protein; DED containing protein FLAME 3; DEFT; DNA binding death effector domain containing protein 2; DNA-binding death effector domain-containing protein 2; FADD like anti apoptotic molecule 3; FLAME 3; anti-DEDD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DEDD2 Antibody detects endogenous levels of DEDD2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DEDD2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DEDD2.
• Conjugation: Unconjugated
• Similarity: Interactions with CASP8 and CASP10 are mediated by the DED domain.
• Subunit Structure: Interacts with CASP8, CASP10 and GTF3C3. Homodimerizes and heterodimerizes with DEDD.
• Tissue Specificity: Expressed in most tissues. High levels were found in liver, kidney, heart, ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Expression was absent or low in colon and small intestine. Expression is relatively high in the tumor cell lines chronic myologenous leukemia K-562 and the colorectal adenocarcinoma SW480. Expression is moderate in the cervical carcinoma HeLa, the Burkitt's lymphoma Raji, the lung carcinoma A-549, and the melanoma G-361. In contrast, two leukemia cell lines, HL-60 (promyelocytic leukemia) and MOLT-4 (lymphoblastic leukemia), show relatively low levels.
• Function: May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.
• Note: Nuclear, accumulated in subnuclear structures resembling nucleoli.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DEDD2 antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 19923050
• NCBI GeneID: 162989
• NCBI Accession #: NP_579874
• NCBI GenBank Nucleotide #: NM_133328.3
• UniProt Accession #: Q8WXF8; Q8NBR2; Q8NES1; Q8TAA8; Q96D35
• Molecular Weight: 35,577 Da
• NCBI Official Full Name: DNA-binding death effector domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: death effector domain containing 2
• NCBI Official Symbol: DEDD2
• NCBI Official Synonym Symbols: FLAME-3
• NCBI Protein Information: DNA-binding death effector domain-containing protein 2; DED-containing protein FLAME-3; FADD-like anti-apoptotic molecule 3
• UniProt Protein Name: DNA-binding death effector domain-containing protein 2
• Protein Family: DNA-binding death effector domain-containing protein
• UniProt Gene Name: DEDD2
• UniProt Synonym Gene Names: FLAME3
• UniProt Entry Name: DEDD2_HUMAN

NCBI Description

This gene encodes a nuclear-localized protein containing a death effector domain (DED). The encoded protein may regulate the trafficking of caspases and other proteins into the nucleus during death receptor-induced apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

DEDD2: May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding; receptor signaling complex scaffold activity; DNA binding

Biological Process: RNA processing; induction of apoptosis via death domain receptors; transcription, DNA-dependent; apoptotic nuclear changes; rRNA catabolic process; cellular homeostasis; negative regulation of transcription, DNA-dependent