Rabbit anti-Human PRDM9 Polyclonal Antibody | anti-PRDM9 antibody

PRDM9 Antibody

Cat. Num. AAA9630186

• Gene Names: PRDM9; PFM6; MSBP3; ZNF899; MEISETZ
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PRDM9; Polyclonal Antibody; PRDM9 Antibody; EC 2.1.1.43; Histone lysine N methyltransferase PRDM9; Histone-lysine N-methyltransferase PRDM9; MEISETZ; PFM6; PR domain containing 9; PR domain containing protein 9; PR domain zinc finger protein 9; PR domain-containing protein 9; PRDM 9; PRDM9_HUMAN; anti-PRDM9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PRDM9 Antibody detects endogenous levels of PRDM9.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRDM9 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PRDM9.
• Conjugation: Unconjugated
• Similarity: The C2H2-type zinc fingers determine the hotspot localization through its binding to specific DNA sequences. Variations in their sequence affect affinity towards DNA-binding motif. Belongs to the class V-like SAM-binding methyltransferase superfamily.
• Post Translational Modifications: Mono-methylated; automethylated. Tri-methylated; automethylated. Mono-methylation is predominant; automethylation is lower and slower than H3 peptide methylation and is in a highest S-adenosyl-L-methionine concentration-dependent. There are two major sites for automethylation at Lys-368 and Lys-374. Lysines can be simultaneously methylated, such as Lys-368(me3)/Lys-372(me1), Lys-368(me1)/Lys-374(me1) and Lys-368(me1)/Lys-372(me1)/Lys-374(me1). Automethylation is an intramolecular (cis) process.
• Subunit Structure: Homodimer Ref.9). Interacts with EHMT2 and CDYL; interaction only takes place when PRDM9 is bound to hotspot DNA. Interacts with CXXC1; this interaction does not link PRDM9-activated recombination hotspot sites with DSB machinery and is not required for the hotspot recognition pathway. Forms a complex with EWSR1, REC8, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).
• Function: Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate (By similarity). During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression (By similarity). During meiotic progression hotspot-bound PRDM9 interacts with several complexes; in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity). In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation (By similarity). Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation (By similarity). In addition performs automethylation (By similarity). Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation (By similarity).
• Note: Localizes in nuclei of pre-leptotene, leptotene, and early to mid-zygotene spermatocytes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PRDM9 antibody

Nucleus; Chromosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 147905620
• NCBI GeneID: 56979
• NCBI Accession #: NP_064612.2
• NCBI GenBank Nucleotide #: NM_020227.3
• UniProt Accession #: Q9NQV7; Q27Q50; B4DX22
• Molecular Weight: 103,376 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
• NCBI Official Synonym Full Names: PR domain 9
• NCBI Official Symbol: PRDM9
• NCBI Official Synonym Symbols: PFM6; MSBP3; ZNF899; MEISETZ
• NCBI Protein Information: histone-lysine N-methyltransferase PRDM9
• UniProt Protein Name: Histone-lysine N-methyltransferase PRDM9
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM9
• UniProt Synonym Gene Names: PFM6
• UniProt Entry Name: PRDM9_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]