Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-PRDM9 antibody RAB-C370 (MBS488820). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of RAB-C370 (MBS488821, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Human anti-Human PRDM9 Monoclonal Antibody | anti-PRDM9 antibody
Anti-PRDM9 [RAB-C370]
Gene Names
PRDM9; PFM6; MSBP3; ZNF899; MEISETZ
Reactivity
Human
Applications
ELISA, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Synonyms
PRDM9; Monoclonal Antibody; Anti-PRDM9 [RAB-C370]; Histone-lysine N-methyltransferase PRDM9; PR domain-containing protein 9; PR domain zinc finger protein 9; Protein-lysine N-methyltransferase PRDM9; [histone H3]-lysine36 N-trimethyltransferase PRDM9; [histone H3]-lysine4 N-trimethyltransferase PRDM9; [histone H3]-lysine9 N-trimethyltransferase PRDM9; [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9; [histone H4]-lysine20 N-methyltransferase PRDM9; EC:2.1.1.-; anti-PRDM9-RAB-C370; PRDM9-A003-1459; anti-PRDM9 antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
Human Fab fragment, kappa
Clone Number
RAB-C370
Specificity
This antibody recognizes PRDM9 (Histone-lysine N-methyltransferase PRDM9). It binds to a folded domain, amino acids 195-385. PRDM9 is a histone methyltransferase that plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination.
Purity/Purification
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PRDM9 antibody
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation-Mass Spectrometry (IP-MS), Chromatin Immunoprecipitation (ChIP)
Application Notes
This antibody has been used and recommended for the following methods: ELISA; IF; IP-MS; ChIP.
Heavy Chain Modification
His-Tagged
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-PRDM9 antibody RAB-C370 (MBS488820). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of RAB-C370 (MBS488821, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-PRDM9 antibody RAB-C370 (MBS488820). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of RAB-C370 (MBS488821, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-PRDM9 antibody
Recombinant monoclonal antibody to PRDM9. Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody RAB-C370.
This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
Product Categories/Family for anti-PRDM9 antibody
References
N/A
Note on publication: More information on this antibody can be found on the website of the Recombinant Antibody Network.
Note on publication: More information on this antibody can be found on the website of the Recombinant Antibody Network.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,376 Da
NCBI Official Full Name
histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
NCBI Official Synonym Full Names
PR domain 9
NCBI Official Symbol
PRDM9
NCBI Official Synonym Symbols
PFM6; MSBP3; ZNF899; MEISETZ
NCBI Protein Information
histone-lysine N-methyltransferase PRDM9
UniProt Protein Name
Histone-lysine N-methyltransferase PRDM9
Protein Family
UniProt Gene Name
PRDM9
UniProt Synonym Gene Names
PFM6
UniProt Entry Name
PRDM9_HUMAN
NCBI Description
The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]
Research Articles on PRDM9
Similar Products
Product Notes
The PRDM9 prdm9 (Catalog #AAA488820) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRDM9 [RAB-C370] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation-Mass Spectrometry (IP-MS), Chromatin Immunoprecipitation (ChIP). This antibody has been used and recommended for the following methods: ELISA; IF; IP-MS; ChIP. Researchers should empirically determine the suitability of the PRDM9 prdm9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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