Rabbit CPSF7 Polyclonal Antibody | anti-CPSF7 antibody

CPSF7 Antibody

Cat. Num. AAA9629869

• Gene Names: CPSF7; CFIm59
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CPSF7; Polyclonal Antibody; CPSF7 Antibody; CFIm59; Cleavage and polyadenylation specificity factor 59 kDa subunit; Cleavage and polyadenylation specificity factor 7; Cleavage and polyadenylation specificity factor subunit 7; CPSF 59 kDa subunit; Cpsf7; CPSF7_HUMAN; FLJ12529 pre mRNA cleavage factor I 59 kDa subunit; FLJ39024; MGC9315; Pre mRNA cleavage factor Im 59 kDa subunit; Pre-mRNA cleavage factor Im 59 kDa subunit; anti-CPSF7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CPSF7 Antibody detects endogenous levels of CPSF7.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CPSF7 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CPSF7.
• Conjugation: Unconjugated
• Similarity: Contains an Arg/Ser-rich domain composed of arginine-serine dipeptide repeats within the C-terminal region that is necessary and sufficient for activating mRNA 3'-processing (PubMed:29276085). Belongs to the RRM CPSF6/7 family.
• Post Translational Modifications: Phosphorylated.Asymmetrically dimethylated on arginine residues by PRMT1.
• Subunit Structure: Component of the cleavage factor Im (CFIm) complex which is a heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or a heterodimer of CPSF6 and CPSF7 Ref.22). The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery. Interacts with NUDT21/CPSF5. Interacts (via Arg/Ser-rich domain) with FIP1L1 (preferentially via unphosphorylated form and Arg/Glu/Asp-rich region); this interaction mediates, at least in part, the interaction between the CFIm and CPSF complexes and may be inhibited by CPSF7 hyper-phosphorylation.
• Function: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF7 activates directly the mRNA 3'-processing machinery. Binds to pA signals in RNA substrates.
• Note: huttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CPSF7 antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 74759932
• NCBI GeneID: 79869
• UniProt Accession #: Q8N684; Q7Z3H9; Q9H025; Q9H9V1; B3KU04; C9K0Q4
• Molecular Weight: 471
• NCBI Official Full Name: Cleavage and polyadenylation specificity factor subunit 7
• NCBI Official Synonym Full Names: cleavage and polyadenylation specific factor 7, 59kDa
• NCBI Official Symbol: CPSF7
• NCBI Official Synonym Symbols: CFIm59
• NCBI Protein Information: cleavage and polyadenylation specificity factor subunit 7; CPSF 59 kDa subunit; cleavage factor Im complex 59 kDa subunit; pre mRNA cleavage factor I, 59 kDa subunit; pre-mRNA cleavage factor I, 59 kDa subunit; pre-mRNA cleavage factor Im 59 kDa subunit
• UniProt Protein Name: Cleavage and polyadenylation specificity factor subunit 7
• Protein Family: Cleavage and polyadenylation specificity factor
• UniProt Gene Name: CPSF7
• UniProt Synonym Gene Names: CFIm59; CPSF 59 kDa subunit
• UniProt Entry Name: CPSF7_HUMAN

Uniprot Description

CPSF7: probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. Probable component of the cleavage factor Im (CFIm) complex, composed at least of NUDT21/CPSF5 and CPSF6 or CPSF7. Two alternatively-spliced isoforms have been described.

Protein type: RNA splicing; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; membrane; nucleus; mRNA cleavage factor complex

Molecular Function: protein binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; protein tetramerization