Testing Data
(Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody (E011185, Red).)
DARPP-32 Antibody | anti-DARPP-32 antibody
DARPP-32 (Phospho-Thr75) Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
DARPP-32; DARPP-32 (Phospho-Thr75) Antibody; anti-DARPP-32 antibody
Reactivity
Human, Mouse, Rat
Specificity
DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
168
Applicable Applications for anti-DARPP-32 antibody
Immunofluorescence (IF)
Application Notes
IF: 1:100-1:200
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
Preparation and Storage
Store at-20 degree C for 1 year
Testing Data
(Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody (E011185, Red).)
Testing Data
(Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody (E011185, Red).)
Product Categories/Family for anti-DARPP-32 antibody
References
Gammie SC. et al.(2008) PLoS ONE. 2008 Apr 9; 3(4):e1974. Alves S. et al. (2008) Hum Mol Genet. 2008 Jul 15; 17(14):2071-83. Bibb, J.A. et al. (1999) Nature 402, 669-671 Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,738 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 1B isoform 2
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 1B
NCBI Official Symbol
PPP1R1B
NCBI Official Synonym Symbols
DARPP32; DARPP-32
NCBI Protein Information
protein phosphatase 1 regulatory subunit 1B
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 1B
UniProt Gene Name
PPP1R1B
UniProt Synonym Gene Names
DARPP32
NCBI Description
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Inhibitor of protein-phosphatase 1.
Research Articles on DARPP-32
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Product Notes
The DARPP-32 ppp1r1b (Catalog #AAA856754) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The DARPP-32 (Phospho-Thr75) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DARPP-32 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:100-1:200. Researchers should empirically determine the suitability of the DARPP-32 ppp1r1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARPP-32, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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