Rabbit anti-Human Legumain Polyclonal Antibody | anti-LGMN antibody
Legumain Antibody
Gene Names
LGMN; AEP; LGMN1; PRSC1
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Legumain; Polyclonal Antibody; Legumain Antibody; AEP; Asparaginyl endopeptidase; cysteine 1; Cysteine protease 1; EC 3.4.22.34; LGMN; LGMN_HUMAN; LGMN1; Protease; Protease cysteine 1 (legumain); Protease cysteine 1; PRSC1; anti-LGMN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Legumain Antibody detects endogenous levels of total Legumain.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LGMN antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
In the zymogen form, the uncleaved propeptide blocks access to the active site. Belongs to the peptidase C13 family.
Post Translational Modifications
Glycosylated.Activated by autocatalytic processing at pH 4.
Subunit Structure
Homodimer before autocatalytic removal of the propeptide (By similarity). Monomer after autocatalytic processing. May interact with integrins.
Tissue Specificity
Ubiquitous. Particularly abundant in kidney, heart and placenta.
Function
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,008 Da
NCBI Official Full Name
legumain preproprotein
NCBI Official Synonym Full Names
legumain
NCBI Official Symbol
LGMN
NCBI Official Synonym Symbols
AEP; LGMN1; PRSC1
NCBI Protein Information
legumain
UniProt Protein Name
Legumain
Protein Family
UniProt Gene Name
LGMN
UniProt Synonym Gene Names
PRSC1
UniProt Entry Name
LGMN_HUMAN
Uniprot Description
LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.
Protein type: EC 3.4.22.34; Protease
Chromosomal Location of Human Ortholog: 14q32.1
Cellular Component: lysosomal lumen; lysosome
Molecular Function: cysteine-type endopeptidase activity; peptidase activity
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process
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Product Notes
The LGMN lgmn (Catalog #AAA9629723) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Legumain Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Legumain can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the LGMN lgmn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Legumain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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