Rabbit RNF7 Polyclonal Antibody | anti-RNF7 antibody

RNF7 Antibody

Cat. Num. AAA9629281

• Gene Names: RNF7; SAG; ROC2; CKBBP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RNF7; Polyclonal Antibody; RNF7 Antibody; CKBBP 1; CKBBP1; CKII beta binding protein 1; CKII beta-binding protein 1; Rbx 2; Rbx2; RBX2_HUMAN; Regulator of cullins 2; RING box protein 2; RING finger protein 7; RING-box protein 2; RNF 7; ROC 2; ROC2; SAG; Sensitive to apoptosis gene; Sensitive to apoptosis gene protein; Zinc RING finger protein SAG; anti-RNF7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RNF7 Antibody detects endogenous levels of total RNF7.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RNF7 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion. Belongs to the RING-box family.
• Post Translational Modifications: Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
• Subunit Structure: Probable part of SCF complexes, which consist of SKP1, CUL1, RNF7/RBX2 and a F-box protein. Interacts (preferentially) with CUL5. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B. Interacts with UBE2F. Interacts with CSNK2B, the interaction is not affected by phosphorylation by CK2.
• Tissue Specificity: Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
• Function: Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RNF7 antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 319004144
• NCBI GeneID: 9616
• NCBI Accession #: NP_001188299.1
• NCBI GenBank Nucleotide #: NM_001201370.1
• UniProt Accession #: Q9UBF6; Q9BXN8; Q9Y5M7; A8K1H9; A8MTB5; C9JYL3; D3DNF7; D3DNF8
• Molecular Weight: 10,876 Da
• NCBI Official Full Name: RING-box protein 2 isoform 4
• NCBI Official Synonym Full Names: ring finger protein 7
• NCBI Official Symbol: RNF7
• NCBI Official Synonym Symbols: SAG; ROC2; CKBBP1
• NCBI Protein Information: RING-box protein 2; CKII beta-binding protein 1; rbx2; regulator of cullins 2; sensitive to apoptosis gene protein; sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
• UniProt Protein Name: RING-box protein 2
• Protein Family: RING-box protein
• UniProt Gene Name: RNF7
• UniProt Synonym Gene Names: RBX2; ROC2; SAG; Rbx2; CKBBP1
• UniProt Entry Name: RBX2_HUMAN

NCBI Description

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF7: a highly conserved ring finger protein. An essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. The RING-type zinc finger recruits the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. This protein interacts with, and is a substrate of, casein kinase II (CKII). The phosphorylation of this protein by CKII has been shown to promote the degradation of IkappaB-alpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Two alternatively spliced isoforms have been reported.

Protein type: Ligase; Motility/polarity/chemotaxis; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 3q22-q24

Cellular Component: nucleoplasm; Cul5-RING ubiquitin ligase complex; cytoplasm; nucleus

Molecular Function: protein binding; copper ion binding; zinc ion binding; NEDD8 ligase activity

Biological Process: protein neddylation; protein ubiquitination; response to redox state; induction of apoptosis by oxidative stress; negative regulation of apoptosis