SERPINB10 blocking peptide

SERPINB10 Blocking Peptide

Cat. Num. AAA9628914

• Gene Names: SERPINB10; PI10; PI-10
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: SERPINB10; SERPINB10 Blocking Peptide; Bomapin; Peptidase inhibitor 10; PI-10; PI10; Protease inhibitor 10; Protease inhibitor 10 (ovalbumin type bomapin); Serpin B10; Serpin peptidase inhibitor clade B (ovalbumin) member 10; SPB10_HUMAN; SERPINB10 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SERPINB10 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SERPINB10 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4826902
• NCBI GeneID: 5273
• NCBI Accession #: NP_005015.1
• NCBI GenBank Nucleotide #: NM_005024.2
• UniProt Accession #: P48595; Q4VAX4; Q4VAX7
• Molecular Weight: 45,403 Da
• NCBI Official Full Name: serpin B10
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade B (ovalbumin), member 10
• NCBI Official Symbol: SERPINB10
• NCBI Official Synonym Symbols: PI10; PI-10
• NCBI Protein Information: serpin B10; bomapin; OTTHUMP00000066999; peptidase inhibitor 10; protease inhibitor 10 (ovalbumin type, bomapin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 10
• UniProt Protein Name: Serpin B10
• Protein Family: Serpin
• UniProt Gene Name: SERPINB10
• UniProt Synonym Gene Names: PI10
• UniProt Entry Name: SPB10_HUMAN

NCBI Description

The superfamily of high molecular weight serine proteinase inhibitors (serpins) regulate a diverse set of intracellular and extracellular processes such as complement activation, fibrinolysis, coagulation, cellular differentiation, tumor suppression, apoptosis, and cell migration. Serpins are characterized by a well-conserved tertiary structure that consists of 3 beta sheets and 8 or 9 alpha helices (Huber and Carrell, 1989 [PubMed 2690952]). A critical portion of the molecule, the reactive center loop connects beta sheets A and C. Protease inhibitor-10 (PI10; SERPINB10) is a member of the ov-serpin subfamily, which, relative to the archetypal serpin PI1 (MIM 107400), is characterized by a high degree of homology to chicken ovalbumin, lack of N- and C-terminal extensions, absence of a signal peptide, and a serine rather than an asparagine residue at the penultimate position (summary by Bartuski et al., 1997 [PubMed 9268635]).[supplied by OMIM]

Uniprot Description

SERPINB10: Protease inhibitor that may play a role in the regulation of protease activities during hematopoiesis and apoptosis induced by TNF. May regulate protease activities in the cytoplasm and in the nucleus. Belongs to the serpin family. Ov-serpin subfamily.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: extracellular space; cytoplasm; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity