Typical Standard Curve/Testing Data
Human Serpin B10 (SERPINB10) ELISA Kit | SERPINB10 elisa kit
Human Serpin B10 (SERPINB10) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: SERPINB10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SERPINB10 antibody and an SERPINB10-HRP conjugate. The assay sample and buffer are incubated together with SERPINB10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERPINB10 concentration since SERPINB10 from samples and SERPINB10-HRP conjugate compete for the anti-SERPINB10 antibody binding site. Since the number of sites is limited, as more sites are occupied by SERPINB10 from the sample, fewer sites are left to bind SERPINB10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERPINB10 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The superfamily of high molecular weight serine proteinase inhibitors (serpins) regulate a diverse set of intracellular and extracellular processes such as complement activation, fibrinolysis, coagulation, cellular differentiation, tumor suppression, apoptosis, and cell migration. Serpins are characterized by a well-conserved tertiary structure that consists of 3 beta sheets and 8 or 9 alpha helices (Huber and Carrell, 1989 [PubMed 2690952]). A critical portion of the molecule, the reactive center loop connects beta sheets A and C. Protease inhibitor-10 (PI10; SERPINB10) is a member of the ov-serpin subfamily, which, relative to the archetypal serpin PI1 (MIM 107400), is characterized by a high degree of homology to chicken ovalbumin, lack of N- and C-terminal extensions, absence of a signal peptide, and a serine rather than an asparagine residue at the penultimate position (summary by Bartuski et al., 1997 [PubMed 9268635]).[supplied by OMIM, Jan 2010]
Uniprot Description
SERPINB10: Protease inhibitor that may play a role in the regulation of protease activities during hematopoiesis and apoptosis induced by TNF. May regulate protease activities in the cytoplasm and in the nucleus. Belongs to the serpin family. Ov-serpin subfamily.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: extracellular space; cytoplasm; nucleus
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on SERPINB10
Similar Products
Product Notes
The Human SERPINB10 serpinb10 (Catalog #AAA7237593) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7237593 ELISA Kit recognizes Human SERPINB10. It is sometimes possible for the material contained within the vial of "Serpin B10 (SERPINB10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
