KCC2 Blocking Peptide | SLC12A5 blocking peptide

KCC2 Blocking Peptide

Cat. Num. AAA9622283

• Gene Names: SLC12A5; KCC2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: KCC2; KCC2 Blocking Peptide; Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; Potassium Chloride Cotransporter; Potassium chloride transporter 5; rKCC2; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter) member 5; Solute carrier family 12 member 5; SLC12A5 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SLC12A5 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SLC12A5 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 161784306
• NCBI GeneID: 57468
• UniProt Accession #: Q9H2X9; Q5VZ41; Q9H4Z0; Q9ULP4; A2RTX2
• Molecular Weight: 1139
• NCBI Official Full Name: Solute carrier family 12 member 5
• NCBI Official Synonym Full Names: solute carrier family 12 (potassium/chloride transporter), member 5
• NCBI Official Symbol: SLC12A5
• NCBI Official Synonym Symbols: KCC2
• NCBI Protein Information: solute carrier family 12 member 5; hKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; erythroid K-Cl cotransporter 2; electroneutral potassium-chloride cotransporter 2
• UniProt Protein Name: Solute carrier family 12 member 5
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC12A5
• UniProt Synonym Gene Names: KCC2; KIAA1176; hKCC2
• UniProt Entry Name: S12A5_HUMAN

NCBI Description

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]

Uniprot Description

KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell soma; integral to plasma membrane; integral to membrane; plasma membrane; dendritic shaft

Molecular Function: protein kinase binding; potassium:chloride symporter activity

Biological Process: response to drug; synaptic transmission; transport; cellular ion homeostasis; multicellular organism growth; ion transport; learning; transmembrane transport; potassium ion transport; thermosensory behavior