JMJD3 blocking peptide

JMJD3 Blocking Peptide

Cat. Num. AAA9621804

• Gene Names: KDM6B; JMJD3
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: JMJD3; JMJD3 Blocking Peptide; Histone demethylase JMJD3; JmjC domain containing protein 3; JmjC domain-containing protein 3; Jumonji D3; Jumonji domain containing 3; Jumonji domain containing 3 histone lysine demethylase; Jumonji domain containing protein 3; Jumonji domain-containing protein 3; Kdm6b; KDM6B_HUMAN; KIAA0346; Lysine demethylase 6B; Lysine K specific demethylase 6B; Lysine specific demethylase 6B; Lysine-specific demethylase 6B; JMJD3 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for JMJD3 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for JMJD3 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 119610532
• NCBI GeneID: 23135
• NCBI Accession #: EAW90126
• NCBI GenBank Nucleotide #: NM_001080424.1
• UniProt Accession #: O15054; Q96G33; C9IZ40
• NCBI Official Full Name: lysine-specific demethylase 6B
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 6B
• NCBI Official Symbol: KDM6B
• NCBI Official Synonym Symbols: JMJD3
• NCBI Protein Information: lysine-specific demethylase 6B; lysine demethylase 6B; jmjC domain-containing protein 3; jumonji domain-containing protein 3; jumonji domain containing 3, histone lysine demethylase
• UniProt Protein Name: Lysine-specific demethylase 6B
• UniProt Gene Name: KDM6B
• UniProt Synonym Gene Names: JMJD3; KIAA0346
• UniProt Entry Name: KDM6B_HUMAN

Uniprot Description

JMJD3: Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Interacts with TLE1. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and KDM6B. By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Belongs to the UTX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; dioxygenase activity; sequence-specific DNA binding; metal ion binding; beta-catenin binding

Biological Process: endothelial cell differentiation; establishment and/or maintenance of chromatin architecture; mesodermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; cardiac muscle cell differentiation