HLAE blocking peptide

HLAE Blocking Peptide

Cat. Num. AAA9620707

• Gene Names: HLA-E; MHC; QA1; EA1.2; EA2.1; HLA-6.2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: HLAE; HLAE Blocking Peptide; HLA class I histocompatibility antigen E alpha chain; EA1.2; EA2.1; HLA 6.2; HLA class I histocompatibility antigen alpha chain E; HLA class I histocompatibility antigen E alpha chain precursor; HLA6.2; Lymphocyte antigen; Major histocompatibility complex class I E; MHC; MHC class I antigen E; MHC HLA E alpha 1; MHC HLA E alpha 2.1; QA1; HLAE blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for HLAE blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for HLAE blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 62912479
• NCBI GeneID: 3133
• NCBI Accession #: NP_005507.3
• NCBI GenBank Nucleotide #: NM_005516.5
• UniProt Accession #: P13747; Q30169; Q9BT83; Q9GIY7; Q9GIY8
• Molecular Weight: 40,157 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain E
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, E
• NCBI Official Symbol: HLA-E
• NCBI Official Synonym Symbols: MHC; QA1; EA1.2; EA2.1; HLA-6.2
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC class I antigen E; lymphocyte antigen
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain E
• UniProt Gene Name: HLA-E
• UniProt Synonym Gene Names: HLA-6.2; HLAE
• UniProt Entry Name: HLAE_HUMAN

NCBI Description

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-E: Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. Belongs to the MHC class I family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; early endosome membrane; plasma membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; MHC class I protein binding; beta-2-microglobulin binding; receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; positive regulation of natural killer cell mediated immunity; regulation of natural killer cell mediated immunity; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of endogenous peptide antigen via MHC class Ib; protection from natural killer cell mediated cytotoxicity