Acinus Blocking Peptide | ACIN1 blocking peptide

Phospho-Acinus (Ser1180) Blocking Peptide

Cat. Num. AAA9619200

• Gene Names: ACIN1; ACN; ACINUS; fSAP152
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: Acinus; Phospho-Acinus (Ser1180) Blocking Peptide; ACIN 1; ACIN1; ACINU_HUMAN; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670; arginine/serine-rich 15; CTD-binding SR-like protein RA4; DKFZp434E098; FLJ23364; KIAA1172; Pre mRNA splicing SR protein rA4; SCAF4; SFR15_HUMAN; Splicing factor; Splicing factor arginine/serine rich 15; Splicing factor serine alanine 15; SR like CTD associated factor 4; SR-related and CTD-associated factor 4; SRA4; CAPER alpha; CAPER; CAPERalpha; CC1.3; Coactivator of activating protein 1 and estrogen receptors; Coactivator of AP1 and ERs; DKFZp781C0423; FLJ44170; HCC 1; Hepatocellular carcinoma protein 1; RBM 39; RBM39; RBM39_HUMAN; RNA binding motif protein 39; RNA binding region (RNP1 RRM) containing 2; RNA binding region containing 2; RNA binding region containing protein 2; RNA-binding motif protein 39; RNA-binding protein 39; RNA-binding region-containing protein 2; RNPC 2; RNPC2; Splicing factor CC1.3; Splicing factor CC1.4; Splicing factor HCC 1; Splicing factor HCC1; Transcription coactivator CAPER; ACIN1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ACIN1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ACIN1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 308153407
• NCBI GeneID: 22985
• UniProt Accession #: Q9UKV3; O95104; Q8TF01; Q14498
• Molecular Weight: 1341
• NCBI Official Full Name: Apoptotic chromatin condensation inducer in the nucleus
• NCBI Official Synonym Full Names: apoptotic chromatin condensation inducer 1
• NCBI Official Symbol: ACIN1
• NCBI Official Synonym Symbols: ACN; ACINUS; fSAP152
• NCBI Protein Information: apoptotic chromatin condensation inducer in the nucleus; functional spliceosome-associated protein 152
• UniProt Protein Name: Apoptotic chromatin condensation inducer in the nucleus
• Protein Family: Apoptotic chromatin condensation inducer in the nucleus
• UniProt Gene Name: ACIN1
• UniProt Synonym Gene Names: ACINUS; KIAA0670; Acinus
• UniProt Entry Name: ACINU_HUMAN

NCBI Description

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

acinus: an ubiquitous RNA-binding protein. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC is composed of ACIN1, CASC3, DDX48, MAGOH, PNN, RBM8A, RNPS1, SAP18 and THOC4. Forms heterodimers with RNPS1. Found in a heterotrimeric complex with ACIN1, RNPS1 and SAP18. Interacts with API5. Phosphorylation by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm, and up-regulates its stimulatory effect on cyclin A1. Activated by proteolytic cleavage. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells. Four alternatively spliced human isoforms have been described.

Protein type: RNA-binding; RNA splicing; Spliceosome; Apoptosis

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; plasma membrane; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; nucleic acid binding; ATPase activity; nucleotide binding

Biological Process: positive regulation of monocyte differentiation; negative regulation of nuclear mRNA splicing, via spliceosome; apoptosis; positive regulation of apoptosis; RNA splicing; erythrocyte differentiation; mRNA processing; apoptotic chromosome condensation; cell structure disassembly during apoptosis