NCoR1 blocking peptide

NCoR1 Blocking Peptide

Cat. Num. AAA9614759

• Gene Names: NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: NCoR1; NCoR1 Blocking Peptide; hN CoR; hNCoR; KIAA1047; N CoR; N Cor/SMRT corepressor Rip13; N CoR1; N-CoR; N-CoR1; NCOR 1; NCoR; Ncor1; NCOR1_HUMAN; Nuclear receptor co repressor 1; Nuclear receptor corepressor 1; Retinoid X receptor interacting protein 13; RIP13; Rxrip13; thyroid hormone and retinoic acid receptor associated corepressor 1; TRAC 1; TRAC1; NCoR1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for NCoR1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NCoR1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 298919179
• NCBI GeneID: 9611
• NCBI Accession #: NP_001177367.1
• NCBI GenBank Nucleotide #: NM_001190438.1
• UniProt Accession #: O75376; Q86YY0; Q9UPV5; Q9UQ18; B3DLF8; E9PGV6
• Molecular Weight: 270,210 Da
• NCBI Official Full Name: nuclear receptor corepressor 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor corepressor 1
• NCBI Official Symbol: NCOR1
• NCBI Official Synonym Symbols: N-CoR; TRAC1; N-CoR1; hN-CoR
• NCBI Protein Information: nuclear receptor corepressor 1; nuclear receptor co-repressor 1; thyroid hormone- and retinoic acid receptor-associated corepressor 1
• UniProt Protein Name: Nuclear receptor corepressor 1
• Protein Family: Nuclear receptor corepressor
• UniProt Gene Name: NCOR1
• UniProt Synonym Gene Names: KIAA1047; N-CoR; N-CoR1
• UniProt Entry Name: NCOR1_HUMAN

NCBI Description

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010]

Uniprot Description

N-CoR1: is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9. Increased expression correlates with the loss of vitamin D responsiveness in aggressive androgen-independent prostate cancer cells. Its gene tends to be overexpressed in multiple-myeloma cell lines.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; Sin3 complex; membrane; spindle microtubule; nuclear chromatin; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein binding; histone deacetylase regulator activity; sequence-specific DNA binding; histone deacetylase binding; thyroid hormone receptor binding; chromatin binding; transcription factor activity; transcription corepressor activity; nuclear hormone receptor binding

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; negative regulation of JNK cascade; CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; regulation of multicellular organism growth; chromatin modification; thalamus development; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; spindle assembly; positive regulation of histone deacetylation; cholesterol homeostasis; transforming growth factor beta receptor signaling pathway; gene expression; circadian regulation of gene expression; negative regulation of phosphoinositide 3-kinase cascade