Rabbit HIPK1/2/3 Polyclonal Antibody | anti-HIPK1/2/3 antibody

Phospho-HIPK1/2/3 (Tyr352/Tyr361/Tyr359) Antibody

Cat. Num. AAA9614156

• Gene Names: HIPK1; Myak; Nbak2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (88%)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: HIPK1/2/3; Polyclonal Antibody; Phospho-HIPK1/2/3 (Tyr352/Tyr361/Tyr359) Antibody; Hipk 1; Hipk1; HIPK1_HUMAN; Homeodomain interacting protein kinase 1; Homeodomain interacting protein kinase 1 like protein; Homeodomain-interacting protein kinase 1; Myak; Nbak 2; Nbak2; Nuclear body associated kinase 2; Nuclear body associated kinase 2b; hHIPk 2; hHIPk2; HIPK 2; Hipk2; HIPK2_HUMAN; Homeodomain interacting protein kinase 2; Homeodomain-interacting protein kinase 2; Nbak1; Nuclear body-associated kinase 1; PRO0593; Sialophorin tail-associated nuclear serine/threonine-protein kinase; Stank; Androgen receptor interacting nuclear protein kinase; Androgen receptor-interacting nuclear protein kinase; ANPK; DYRK6; Fas interacting serine/threonine protein kinase; Fas-interacting serine/threonine-protein kinase; FIST; FIST3; Hipk3; HIPK3_HUMAN; homeodomain interacting protein kinase 3; Homeodomain-interacting protein kinase 3; Homolog of protein kinase YAK1; PKY; RP24 297H17.5; YAK1; anti-HIPK1/2/3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (88%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-HIPK1/2/3 (Tyr352/Tyr361/Tyr359) Antibody detects endogenous levels of HIPK1/2/3 only when phosphorylated at Tyr352/361/359.; Tissue Specificity: Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HIPK1/2/3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human HIPK1/2/3 around the phosphorylation site of Tyr352/361/359.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated, but autophosphorylation is not required for catalytic activity.May be sumoylated.
• Subunit Structure: Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with and stabilizes ligand-bound androgen receptor (AR) (By similarity). Interacts with UBL1/SUMO-1. Binds to NR5A1/SF1, SPEN/MINT and RUNX2.
• Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
• Subcellular Location: Cytoplasm. Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using Phospho-HIPK1/2/3 (Tyr352/Tyr361/Tyr359) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: HepG2 cells(heat shock treatment).)

Western Blot (WB)

(Western blot analysis of HIPK1/2/3 (Phospho-Tyr352/361/359) using TNF-α treated A2780 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Immunohistochemistry (IHC)

(At 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/200 staining Mouse ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-HIPK1/2/3 antibody

Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).

NCBI and Uniprot Product Information

• NCBI GI #: 38201638
• NCBI GeneID: 204851
• NCBI Accession #: NP_689909.2
• NCBI GenBank Nucleotide #: NM_152696.3
• UniProt Accession #: Q86Z02; Q9H2X6; Q9H422
• Molecular Weight: 87,302 Da
• NCBI Official Full Name: homeodomain-interacting protein kinase 1 isoform 2
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 1
• NCBI Official Symbol: HIPK1
• NCBI Official Synonym Symbols: Myak; Nbak2
• NCBI Protein Information: homeodomain-interacting protein kinase 1; nuclear body-associated kinase 2; nuclear body associated kinase 2b; homeodomain interacting protein kinase 1-like protein
• UniProt Protein Name: Homeodomain-interacting protein kinase 1
• UniProt Gene Name: HIPK1
• UniProt Synonym Gene Names: KIAA0630; MYAK; NBAK2
• UniProt Entry Name: HIPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

HIPK1: homeodomain interacting protein kinase 1 is a CMGC kinase of the DYRK family. May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Expression elevated in many breast cancer cell lines. Null mice are resistant to carcinogens. Binds the p53 tumor suppressor. 4 alternatively spliced isoforms of the human protein have been described.

Protein type: Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; DYRK family; HIPK subfamily

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: PML body; cytoplasm; nuclear speck; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: neuron differentiation; anterior/posterior pattern formation; positive regulation of angiogenesis; smoothened signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; eye development; embryonic retina morphogenesis in camera-type eye; positive regulation of cell proliferation; embryonic camera-type eye morphogenesis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein amino acid phosphorylation