Rabbit anti-Human EPRS Polyclonal Antibody | anti-EPRS antibody

EPRS Antibody

Cat. Num. AAA9613759

• Gene Names: EPRS; EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: EPRS; Polyclonal Antibody; EPRS Antibody; Bifunctional aminoacyl tRNA synthetase; Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; Eprs; GLNS; Glu pro tRNA synthetase; GLUPRORS; GluRS; Glutamate tRNA ligase; Glutamatyl prolyl tRNA synthetase; Glutaminyl tRNA synthetase; Glutamyl prolyl tRNA synthetase; Glutamyl tRNA synthetase; Glutamyl-tRNA synthetase; PARS; PIG 32; PIG32; Proliferation inducing gene 32 protein; Proliferation inducing protein 32; Proline tRNA ligase; Proline--tRNA ligase; Prolyl tRNA synthetase; Prolyl-tRNA synthetase; QARS; QPRS; SYEP_HUMAN; anti-EPRS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: EPRS Antibody detects endogenous levels of total EPRS.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EPRS antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human EPRS.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated at Ser-886 by CDK5. Phosphorylated at Ser-999 by RPS6KB1; triggers EPRS1 release from the aminoacyl-tRNA synthetase multienzyme complex. In monocytes, the IFN-gamma-induced sequential phosphorylation at Ser-886 and Ser-999 releases EPRS1 from the aminoacyl-tRNA synthetase multienzyme complex, allowing its association with the GAIT complex. Phosphorylation at Ser-999 is specifically required for the RPL13A-mediated interaction of the GAIT complex with eIF4G. Phosphorylation at Ser-999 by RPS6KB1, is also induced by insulin through activation of the mTORC1 signaling pathway and promotes the interaction of EPRS1 with SLC27A1.
• Subunit Structure: Homodimer. Part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex (MSC), that is composed of the tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex. Interacts with TARS3. Interacts with DUS2L. Component of the GAIT complex which is composed of EPRS1, RPL13A and GAPDH. For human, the complex assembly seems to be a two-step process in which EPRS1 first associates with SYNCRIP to form a pre-GAIT complex which is deficient in GAIT element binding. Interacts (phosphorylated at Ser-999) with SLC27A1; mediates the translocation of SLC27A1 from the cytoplasm to the plasma membrane thereby increasing the uptake of long-chain fatty acids.
• Similarity: The WHEP-TRS domains are involved in RNA binding.In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
• Subcellular Location: Cytoplasm>Cytosol. Membrane>Peripheral membrane protein.; Note: Translocates from cytosol to membranes upon phosphorylation at Ser-999.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-EPRS antibody

Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan.

NCBI and Uniprot Product Information

• NCBI GI #: 62241042
• NCBI GeneID: 2058
• NCBI Accession #: NP_004437.2
• NCBI GenBank Nucleotide #: NM_004446.2
• UniProt Accession #: P07814; Q05BP6; Q05DF8; Q5DSM1; Q5H9S5; Q6PD57; Q86X73; A0AVA9; B9EGH3
• Molecular Weight: 170,591 Da
• NCBI Official Full Name: bifunctional glutamate/proline--tRNA ligase
• NCBI Official Synonym Full Names: glutamyl-prolyl-tRNA synthetase
• NCBI Official Symbol: EPRS
• NCBI Official Synonym Symbols: EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
• NCBI Protein Information: bifunctional glutamate/proline--tRNA ligase
• UniProt Protein Name: Bifunctional glutamate/proline--tRNA ligase
• UniProt Gene Name: EPRS
• UniProt Synonym Gene Names: GLNS; PARS; QARS; QPRS; GluRS

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.