anti-Human Complement C3 Antibody | anti-C3 antibody

Complement C3 (betachain) Antibody

Cat. Num. AAA850641

• Gene Names: C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• Reactivity: Human
• Applications: Western Blot
• Synonyms: Complement C3; Complement C3 (betachain) Antibody; anti-C3 antibody

• Reactivity: Human
• Clone Number: 11-10
• Specificity: This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to the residues of Complement C3.
• Form/Format: Liquid; 1*TBS (pH7.4), 0.5% BSA, 25% Glycerol.; Preservative: 0.05% Sodium Azide.
• Concentration: 0.37mg/ml (varies by lot)
• Sequence Length: 1663

• Applicable Applications for anti-C3 antibody: Western Blot (WB)
• Application Notes: WB: 1:2,000
• Preservative: 0.05% Sodium Azide.
• Cellular Localization: Secretory Protein
• Positive Control: Human plasma
• Preparation and Storage: Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles.

Western blot (WB)

(Western blot analysis on human serum using anti- Complement C3 mouse monoclonal antibody.)

Related Product Information for anti-C3 antibody

Human Complement C3 (C3) is synthesized as a single-chain pro-molecule (185 kDa) that tsuffers several posttranslational modifications. Before being a mature protein, C3 is split into beta-chain (70 kDa) and alpha chain (115 kDa) and forms a rare internal thioester bond. C3 plays a central role in the activation of the complement system. It’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

References

"Structure of C3b reveals conformational changes that underlie complement activity."Janssen B.J.C., Christodoulidou A., McCarthy A., Lambris J.D., Gros P.Nature 444:213-216(2006). "Disulfide bridges in human complement component C3b."Dolmer K., Sottrup-Jensen L.FEBS Lett. 315:85-90(1993). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 115298678
• NCBI GeneID: 718
• NCBI Accession #: NP_000055.2
• NCBI GenBank Nucleotide #: NM_000064.3
• UniProt Accession #: P01024; A7E236
• Molecular Weight: 70kDa
• NCBI Official Full Name: complement C3 preproprotein
• NCBI Official Synonym Full Names: complement C3
• NCBI Official Symbol: C3
• NCBI Official Synonym Symbols: ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• NCBI Protein Information: complement C3
• UniProt Protein Name: Complement C3
• Protein Family: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: CPAMD1; C3bc; ASP

NCBI Description

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]

Uniprot Description

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.