Rabbit GEF H1 Polyclonal Antibody | anti-GEFH1 antibody

Phospho-GEF H1 (Ser886) Antibody

Cat. Num. AAA9613283

• Gene Names: ARHGEF2; GEF; P40; GEFH1; LFP40; GEF-H1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: GEF H1; Polyclonal Antibody; Phospho-GEF H1 (Ser886) Antibody; AA408978; ARHG2; ARHG2_HUMAN; ARHGEF 2; ARHGEF-2; ARHGEF2; GEF; GEF-H1; GEFH1; Guanine nucleotide exchange factor H1; KIAA0651; Lbcl1; Lfc; LFP40; MGC95068; Microtubule-regulated Rho-GEF; mKIAA0651; P40; Proliferating cell nucleolar antigen p40; Protein GEF-H1; Rho guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor (GEF) 2; rho/rac guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor; anti-GEFH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-GEF H1 (Ser886) Antibody detects endogenous levels of GEF H1 only when phosphorylated at Ser886.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GEFH1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human GEF H1 around the phosphorylation site of Ser886.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation of Ser-886 by PAK1 induces binding to protein YWHAZ, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by AURKA and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. Phosphorylated on serine, threonine and tyrosine residues in a RIPK2-dependent manner.
• Subunit Structure: Found in a complex composed at least of ARHGEF2, NOD2 and RIPK2. Interacts with RIPK2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Interacts with RIPK1 and RIPK3. Interacts with YWHAZ/14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA and MAPK1. Interacts with RHOA and RAC1. Interacts with NOD1. Interacts (via the N-terminal zinc finger) with CAPN6 (via domain II). Interacts with DYNLT1 (By similarity).
• Similarity: The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. Promotes tyrosine phosphorylation of RIPK2.The pHdomain has no affinity for phosphoinositides suggesting that it does not interact directly with membranes.The phorbol-ester/DAG-type zinc-finger and the C-terminal coiled-coil domains (606-986) are both important for association with microtubules.
• Subcellular Location: Cytoplasm>Cytoskeleton. Cytoplasm. Cell junction>Tight junction. Golgi apparatus. Cytoplasm>Cytoskeleton>Spindle. Cell projection>Ruffle membrane. Cytoplasmic vesicle.; Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085). Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560). Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using Phospho-GEF H1 (Ser886) Antibody.Lane 1: Hela cells(1hUV treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(1hUV treatment),Lane 3: K562 cells(serum starvation treatment).)

Western Blot (WB)

(Western blot analysis of extracts from 293T, using Phospho-GEF-H1 (Ser886) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Immunohistochemistry (IHC)

(At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-GEFH1 antibody

Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 253735775
• NCBI GeneID: 9181
• NCBI Accession #: NP_001155855.1
• NCBI GenBank Nucleotide #: NM_001162383.1
• UniProt Accession #: Q92974; O75142; Q15079; Q5VY92; Q8TDA3; Q8WUG4; Q9H023; D3DVA6
• Molecular Weight: 111,543 Da
• NCBI Official Full Name: rho guanine nucleotide exchange factor 2 isoform 1
• NCBI Official Synonym Full Names: Rho/Rac guanine nucleotide exchange factor (GEF) 2
• NCBI Official Symbol: ARHGEF2
• NCBI Official Synonym Symbols: GEF; P40; GEFH1; LFP40; GEF-H1
• NCBI Protein Information: rho guanine nucleotide exchange factor 2; microtubule-regulated Rho-GEF; guanine nucleotide exchange factor H1; proliferating cell nucleolar antigen p40; Rho/Rac guanine nucleotide exchange factor 2
• UniProt Protein Name: Rho guanine nucleotide exchange factor 2
• UniProt Gene Name: ARHGEF2
• UniProt Synonym Gene Names: KIAA0651; LFP40; GEF-H1
• UniProt Entry Name: ARHG2_HUMAN

NCBI Description

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Uniprot Description

ARHGEF2: a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases. Mediates cross-talk between microtubules and the actin cytoskeleton. Contains a phorbol esters/diacylglycerol binding and PH domain.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: Golgi apparatus; microtubule; protein complex; tight junction; cytoskeleton; focal adhesion; cell soma; cytoplasmic membrane-bound vesicle; cytoplasm; spindle; dendritic shaft; cytosol; vesicle

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; Rho GTPase binding; zinc ion binding; Rac guanyl-nucleotide exchange factor activity; microtubule binding; Rac GTPase binding; transcription factor binding

Biological Process: mitosis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of microtubule depolymerization; cell morphogenesis; positive regulation of interleukin-6 production; actin filament organization; positive regulation of tumor necrosis factor production; regulation of cell proliferation; negative regulation of neurogenesis; activation of NF-kappaB transcription factor; intracellular protein transport; regulation of small GTPase mediated signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; establishment of mitotic spindle orientation; cell division; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; innate immune response; positive regulation of transcription from RNA polymerase II promoter