Rabbit PP2A-alpha Polyclonal Antibody | anti-PP2A-alpha antibody

Phospho-PP2A-alpha (Tyr307) Antibody

Cat. Num. AAA9613254

• Gene Names: PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PP2A-alpha; Polyclonal Antibody; Phospho-PP2A-alpha (Tyr307) Antibody; PP2A alpha; PP2AA_HUMAN; PP2Aalpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform; Protein phosphatase 2 catalytic subunit alpha isoform; Protein phosphatase 2; catalytic subunit; alpha isozyme; Protein phosphatase 2A catalytic subunit; alpha isoform; Replication protein C; RP C; RP-C; RPC; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; anti-PP2A-alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PP2A-alpha (Tyr307) Antibody detects endogenous levels of PP2A-alpha only when phosphorylated at Tyr307.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PP2A-alpha antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.Polyubiquitinated, leading to its degradation by the proteasome.
• Subunit Structure: PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36kDa catalytic subunit (subunit C) and PPP2R1A a 65kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct (By similarity). Interacts with KCTD20 (By similarity). Interacts with BTBD10 (By similarity). Interacts with SGO1 and SGO2. Interacts with TP53. Interacts with AXIN1; the interaction dephosphorylates AXIN1. Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3. Interacts with RAF1. Interaction with IGBP1 protects unassembled PPP2CA from degradative ubiquitination. Interacts with GSK3B (via C2 domain). Interacts with MFHAS1; retains PPP2CA into the cytoplasm and excludes it from the nucleus. Interacts with FAM122A. Interacts with ADCY8; interaction is phosphatase activity-dependent; antagonizes interaction between ADCY8 and calmodulin. Interacts with CRTC3 (when phosphorylated at 'Ser-391').
• Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily.
• Subcellular Location: Cytoplasm. Nucleus. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle pole.; Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGO2 (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from K562 cells(UV treatment), using Phospho-PP2A-alpha (Tyr307) Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa  , using Phospho-PP2A-alpha (Tyr307) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Related Product Information for anti-PP2A-alpha antibody

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

NCBI and Uniprot Product Information

• NCBI GI #: 4506017
• NCBI GeneID: 5515
• NCBI Accession #: NP_002706.1
• NCBI GenBank Nucleotide #: NM_002715.2
• UniProt Accession #: P67775; P05323; P13197
• Molecular Weight: 35,594 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP2CA
• NCBI Official Synonym Symbols: RP-C; PP2Ac; PP2CA; PP2Calpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; replication protein C; protein phosphatase 2A catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; serine/threonine pr
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha; RP-C
• UniProt Entry Name: PP2AA_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process