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Western Blot (WB) (Western blot analysis of extracts from cos-7, using Phospho-PABP2(Ser150) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Rabbit PABP2 Polyclonal Antibody | anti-PABP2 antibody

Phospho-PABP2 (Ser150) Antibody

Gene Names
PABPN1; OPMD; PAB2; PABII; PABP2; PABP-2
Reactivity
Human, Mouse, Rat, Bovine
Predicted Reactivity: Pig (100%), Zebrafish (80%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
PABP2; Polyclonal Antibody; Phospho-PABP2 (Ser150) Antibody; Nuclear poly (A)-binding protein 1; OPMD; PAB2; PABII; PABP 2; pABP-2; PABP2_HUMAN; PABPII; Pabpn1; poly (A) binding protein nuclear 1; Poly (A)-binding protein 2; Poly (A)-binding protein II; PolyA binding protein II; Polyadenylate-binding nuclear protein 1; Polyadenylate-binding protein 2; anti-PABP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Predicted Reactivity: Pig (100%), Zebrafish (80%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-PABP2 (Ser150) Antibody detects endogenous levels of PABP2 only when phosphorylated at Ser150.
Tissue Specificity: Ubiquitous.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PABP2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human PABP2 around the phosphorylation site of Ser150.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).
Subunit Structure
May interact with SETX. Monomer and homooligomer. Binds RNA as a monomer and oligomerizes when bound to poly (A). Interacts with PAPOLA, but only in presence of oligo (A) RNA. Interacts with transportin. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Association in a ternary complex with CPSF4 and influenza A virus NS1 blocks pre-mRNAs processing, thereby preventing nuclear export of host cell mRNAs. Associates in a single complex with SKIP and MYOD1 and interacts with SKIP in differentiated myocytes. Interacts with NUDT21/CPSF5. Interacts (via RRM domain and C-terminal arginine-rich region) with ZFP36 (via hypophosphorylated form); this interaction occurs in the nucleus in a RNA-independent manner, decreases in presence of single-stranded poly (A) RNA-oligomer and in a p38-dependent-manner and may down-regulated RNA poly (A) polymerase activity (By similarity). Component of the poly (A) tail exosome targeting (PAXT) complex made of accessory factors, such as PABPN1, ZFC3H1 and MTREX. Interacts with ZFC3H1 in a RNase-insensitive manner.
Similarity
The RRM domain is essential for specific adenine bases recognition in the poly (A) tail but not sufficient for poly (A) binding.
Subcellular Location
Nucleus. Cytoplasm. Nucleus speckle.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363). Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism (By similarity). Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly (A) RNA in nuclear speckles (By similarity). Intranuclear filamentous inclusions or 'aggregates' are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly (A) RNA.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from cos-7, using Phospho-PABP2(Ser150) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from cos-7, using Phospho-PABP2(Ser150) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Immunohistochemistry (IHC)

(Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Testing Data

(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Testing Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Related Product Information for anti-PABP2 antibody
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly (A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly (A) polymerase (PAPOLA) conferring processivity on the poly (A) tail elongation reaction and controls also the poly (A) tail length (By similarity). Increases the affinity of poly (A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly (A) and to poly (G) with high affinity (By similarity). May protect the poly (A) tail from degradation (By similarity). Subunit of the trimeric poly (A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly (A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32749 MW
NCBI Official Full Name
polyadenylate-binding protein 2
NCBI Official Synonym Full Names
poly(A) binding protein nuclear 1
NCBI Official Symbol
PABPN1
NCBI Official Synonym Symbols
OPMD; PAB2; PABII; PABP2; PABP-2
NCBI Protein Information
polyadenylate-binding protein 2
UniProt Protein Name
Polyadenylate-binding protein 2
UniProt Gene Name
PABPN1
UniProt Synonym Gene Names
PAB2; PABP2; PABP-2; Poly(A)-binding protein 2; PABII

NCBI Description

This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (). Increases the affinity of poly(A) polymerase for RNA (). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (). May protect the poly(A) tail from degradation (). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).

Research Articles on PABP2

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Product Notes

The PABP2 pabpn1 (Catalog #AAA9613141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PABP2 (Ser150) Antibody reacts with Human, Mouse, Rat, Bovine Predicted Reactivity: Pig (100%), Zebrafish (80%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's PABP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PABP2 pabpn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PABP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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