Western Blot (WB)
(Western blot analysis of extracts from EC304 cells, using Slap Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Slap Polyclonal Antibody | anti-SLA antibody
Slap Antibody
Gene Names
SLA; SLA1; SLAP
Reactivity
Human, Mouse, RatPredicted Reactivity: Bovine(100%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Slap; Polyclonal Antibody; Slap Antibody; hSLAP; SLA 1; sla; SLA1; SLAP 1; SLAP; SLAP-1; SLAP1; SLAP1_HUMAN; Src like adapter; Src like adapter protein 1; Src like adaptor; SRC-like-adapter; Src-like-adapter protein 1; anti-SLA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Sheep(100%), Dog(100%)
Predicted Reactivity: Bovine(100%), Sheep(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Slap Antibody detects endogenous levels of total Slap.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-SLA antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Slap, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from EC304 cells, using Slap Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from EC304 cells, using Slap Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SLA antibody
Function: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.
Subcellular Location: Cytoplasm. Endosome. Note: Colocalizes with endosomes.
Tissue Specificity: Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.
Subunit Structure: Interacts with EPHA2, VAV1, LCP2 and PDGFRB (By similarity). Homodimer. Homodimerization and interaction with phosphorylated CBL occurs via its C-terminal domain. Interacts with phosphorylated proteins ZAP70, CD3Z, SYK and LAT via its SH2 domain.
Similarity: The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization.
Subcellular Location: Cytoplasm. Endosome. Note: Colocalizes with endosomes.
Tissue Specificity: Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.
Subunit Structure: Interacts with EPHA2, VAV1, LCP2 and PDGFRB (By similarity). Homodimer. Homodimerization and interaction with phosphorylated CBL occurs via its C-terminal domain. Interacts with phosphorylated proteins ZAP70, CD3Z, SYK and LAT via its SH2 domain.
Similarity: The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)31kD.
Predicted Molecular Weight: (Calculated)31kDa.
Predicted Molecular Weight: (Calculated)31kDa.
NCBI Official Full Name
src-like-adapter isoform c
NCBI Official Synonym Full Names
Src like adaptor
NCBI Official Symbol
SLA
NCBI Official Synonym Symbols
SLA1; SLAP
NCBI Protein Information
src-like-adapter
UniProt Protein Name
Src-like-adapter
Protein Family
UniProt Gene Name
SLA
UniProt Synonym Gene Names
SLAP; SLAP1; SLAP-1; hSLAP
UniProt Entry Name
SLAP1_HUMAN
Uniprot Description
SLAP: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 8q24
Cellular Component: endosome
Molecular Function: protein binding; SH3/SH2 adaptor activity
Biological Process: positive regulation of signal transduction
Research Articles on SLA
Similar Products
Product Notes
The SLA sla (Catalog #AAA9612203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Slap Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Bovine(100%), Sheep(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Slap can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SLA sla for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Slap, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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