Rabbit anti-Human Src-like Adaptor Polyclonal Antibody | anti-SLA antibody

Src-like Adaptor (Src-like-adapter, SLA, SLAP, hSLAP, Src-like-adapter Protein 1, SLA1, SLAP1, SLAP-1)

Cat. Num. AAA649816

• Gene Names: SLA; SLA1; SLAP
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Src-like Adaptor; Polyclonal Antibody; Src-like Adaptor (Src-like-adapter; SLA; SLAP; hSLAP; Src-like-adapter Protein 1; SLA1; SLAP1; SLAP-1); Anti -Src-like Adaptor (Src-like-adapter; anti-SLA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLAP. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-SLA antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to aa175-225 of human SLAP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of SLAP in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and (bottom) at 5ug/ml.)

Related Product Information for anti-SLA antibody

SLAP also known as Src-like-adapter protein 1 is a 276aa containing adapter protein, functioning as a negative regulator of T-cell receptor (TCR) signaling. SLAP belonging to the SRC family of cytoplasmic tyrosine kinases contains a SH2 domain and a SH3 domain. This cytoplasmic protein colocalizes with endosomes and is known to inhibit T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. It acts as a negative regulator of positive selection and mitosis of T-cells. SLAP may act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. Expression of SLAP is weak in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas but is high in lung and fetal brain.

Product Categories/Family for anti-SLA antibody

Antibodies; Abs to Adapter Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119612565
• NCBI GeneID: 6503
• UniProt Accession #: Q13239; Q9UMQ8
• Molecular Weight: 31,156 Da
• NCBI Official Full Name: Src-like-adaptor
• NCBI Official Synonym Full Names: Src-like-adaptor
• NCBI Official Symbol: SLA
• NCBI Official Synonym Symbols: SLA1; SLAP
• NCBI Protein Information: src-like-adapter; hSLAP; SLAP-1; src-like-adapter protein 1
• UniProt Protein Name: Src-like-adapter
• Protein Family: S-layer protein
• UniProt Gene Name: SLA
• UniProt Synonym Gene Names: SLAP; SLAP1; SLAP-1; hSLAP
• UniProt Entry Name: SLAP1_HUMAN

Uniprot Description

Function: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. Ref.4 Ref.7

Subunit structure: Interacts with EPHA2, VAV1, LCP2 and PDGFRB

By similarity. Homodimer. Homodimerization and interaction with phosphorylated CBL occurs via its C-terminal domain. Interacts with phosphorylated proteins ZAP70, CD3Z, SYK and LAT via its SH2 domain. Ref.7

Subcellular location: Cytoplasm

By similarity. Endosome

By similarity. Note: Colocalizes with endosomes

By similarity.

Tissue specificity: Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas. Ref.3

Induction: By all-trans retinoic acid (ATRA). Induction is indirect and is mediated through other proteins. Ref.2

Domain: The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization.

Sequence similarities: Contains 1 SH2 domain.Contains 1 SH3 domain.