Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using SLC26A4 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit SLC26A4 Polyclonal Antibody | anti-SLC26A4 antibody
SLC26A4 Antibody
Gene Names
SLC26A4; EVA; PDS; DFNB4; TDH2B
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SLC26A4; Polyclonal Antibody; SLC26A4 Antibody; deafness; autosomal recessive 4; DFNB4; EVA; NSRD4; PDS; Pendred syndrome; Pendred syndrome homolog; Pendrin; S26A4_HUMAN; Sodium independent chloride/iodide transporter; Sodium-independent chloride/iodide transporter; Solute carrier family 26 member 4; anti-SLC26A4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(100%)
Predicted Reactivity: Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
SLC26A4 Antibody detects endogenous levels of total SLC26A4.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
780
Applicable Applications for anti-SLC26A4 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SLC26A4, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using SLC26A4 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using SLC26A4 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612168 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612168 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612168 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612168 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Related Product Information for anti-SLC26A4 antibody
Function: Sodium-independent transporter of chloride and iodide.
Subcellular Location: Membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Note: Localizes to the apical brush border of cells in the cortical collecting ducts of the kidney.
Tissue Specificity: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.
Similarity: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
Subcellular Location: Membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Note: Localizes to the apical brush border of cells in the cortical collecting ducts of the kidney.
Tissue Specificity: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.
Similarity: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)85kD.
Predicted Molecular Weight: (Calculated)86kDa.
Predicted Molecular Weight: (Calculated)86kDa.
NCBI Official Full Name
pendrin
NCBI Official Synonym Full Names
solute carrier family 26 member 4
NCBI Official Symbol
SLC26A4
NCBI Official Synonym Symbols
EVA; PDS; DFNB4; TDH2B
NCBI Protein Information
pendrin
UniProt Protein Name
Pendrin
Protein Family
UniProt Gene Name
SLC26A4
UniProt Synonym Gene Names
PDS
NCBI Description
Mutations in this gene are associated with Pendred syndrome, the most common form of syndromic deafness, an autosomal-recessive disease. It is highly homologous to the SLC26A3 gene; they have similar genomic structures and this gene is located 3' of the SLC26A3 gene. The encoded protein has homology to sulfate transporters. [provided by RefSeq, Jul 2008]
Uniprot Description
Sodium-independent transporter of chloride and iodide.
Research Articles on SLC26A4
Similar Products
Product Notes
The SLC26A4 slc26a4 (Catalog #AAA9612168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC26A4 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's SLC26A4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SLC26A4 slc26a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC26A4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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