Human Pendrin ELISA Kit | SLC26A4 elisa kit

Human Pendrin ELISA Kit

Cat. Num. AAA764789

• Gene Names: SLC26A4; EVA; PDS; DFNB4; TDH2B
• Reactivity: Human
• Synonyms: Pendrin; Human Pendrin ELISA Kit; SLC26A4/Pendrin/PDS/Sodium-independent chloride/iodide transporter/Solute carrier family 26 member 4; SLC26A4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Slc25a5. No significant cross-reactivity or interference between Slc25a5 and analogues was observed.
• Sequence Length: 780

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slc25a5 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slc25a5 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC26A4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Slc25a5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Slc25a5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Slc25a5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Slc25a5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505697
• NCBI GeneID: 5172
• NCBI Accession #: NP_000432.1
• NCBI GenBank Nucleotide #: NM_000441.1
• Molecular Weight: 39,267 Da
• NCBI Official Full Name: pendrin
• NCBI Official Synonym Full Names: solute carrier family 26 member 4
• NCBI Official Symbol: SLC26A4
• NCBI Official Synonym Symbols: EVA; PDS; DFNB4; TDH2B
• NCBI Protein Information: pendrin
• UniProt Protein Name: Pendrin
• Protein Family: Pendrin
• UniProt Gene Name: SLC26A4
• UniProt Synonym Gene Names: PDS
• UniProt Entry Name: S26A4_HUMAN

NCBI Description

Mutations in this gene are associated with Pendred syndrome, the most common form of syndromic deafness, an autosomal-recessive disease. It is highly homologous to the SLC26A3 gene; they have similar genomic structures and this gene is located 3' of the SLC26A3 gene. The encoded protein has homology to sulfate transporters. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC26A4: Sodium-independent transporter of chloride and iodide. Defects in SLC26A4 are a cause of Pendred syndrome (PDS). PDS is an autosomal recessive disorder characterized by congenital sensorineural hearing loss combined with thyroid goiter. The disorder may account for up to 10% of the cases of hereditary deafness. The deafness is most often associated with a Mondini cochlear defect. Defects in SLC26A4 are the cause of deafness autosomal recessive type 4 (DFNB4); also known as vestibular aqueduct syndrome (EVA). DFNB4 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNB4 is associated with an enlarged vestibular aqueduct. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: apical plasma membrane; brush border membrane; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: anion:anion antiporter activity; bicarbonate transmembrane transporter activity; chloride channel activity; chloride transmembrane transporter activity; iodide transmembrane transporter activity; oxalate transmembrane transporter activity; secondary active sulfate transmembrane transporter activity; sulfate transmembrane transporter activity

Biological Process: bicarbonate transport; chloride transport; inorganic anion transport; iodide transport; ion transport; oxalate transport; regulation of intracellular pH; regulation of membrane potential; regulation of pH; regulation of protein localization; sensory perception of sound; sulfate transport; transmembrane transport

Disease: Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct; Pendred Syndrome