Western Blot (WB)
(Western blot analysis of extracts from Pc12, using CCDC53 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit CCDC53 Polyclonal Antibody | anti-CCDC53 antibody
CCDC53 Antibody
Gene Names
WASHC3; CCDC53; CGI-116
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
CCDC53; Polyclonal Antibody; CCDC53 Antibody; AD-016; CCD53_HUMAN; CGI-116; Coiled-coil domain-containing protein 53; WASH complex subunit CCDC53; anti-CCDC53 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
CCDC53 Antibody detects endogenous levels of CCDC53.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
194
Applicable Applications for anti-CCDC53 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human CCDC53.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Endosome
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Pc12, using CCDC53 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Pc12, using CCDC53 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human liver tissue, using CCDC53 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human liver tissue, using CCDC53 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CCDC53 antibody
Description: Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093).
Subunit Structure: Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH (WASHC1, WASH2P or WASH3P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. The WASH core complex associates via WASHC2 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex.
Similarity: Belongs to the CCDC53 family.
Subunit Structure: Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH (WASHC1, WASH2P or WASH3P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. The WASH core complex associates via WASHC2 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex.
Similarity: Belongs to the CCDC53 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 21 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
WASH complex subunit 3 isoform 1
NCBI Official Synonym Full Names
WASH complex subunit 3
NCBI Official Symbol
WASHC3
NCBI Official Synonym Symbols
CCDC53; CGI-116
NCBI Protein Information
WASH complex subunit 3
UniProt Protein Name
WASH complex subunit CCDC53
Protein Family
UniProt Gene Name
CCDC53
UniProt Synonym Gene Names
AD-016; CGI-116; x0009
UniProt Entry Name
CCD53_HUMAN
Similar Products
Product Notes
The CCDC53 ccdc53 (Catalog #AAA9611636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCDC53 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CCDC53 ccdc53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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