Rabbit ASNA1 Polyclonal Antibody | anti-ASNA1 antibody

ASNA1 Antibody

Cat. Num. AAA9611314

• Gene Names: GET3; ARSA1; ASNA1; TRC40; ARSA-I; ASNA-I
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ASNA1; Polyclonal Antibody; ASNA1 Antibody; ARSA 1; ARSA; ArsA arsenite transporter ATP binding homolog 1; ArsA arsenite transporter; ATP binding; E. coli; homolog of; 1; ATP-binding; homolog 1 (bacterial); ARSA I; ARSA-I; ARSA1; ARSAI; Arsenical pump driving ATPase; Arsenical pump-driving ATPase; Arsenical resistance ATPase; Arsenite translocating ATPase; Arsenite transporting ATPase; Arsenite-stimulated ATPase; ASNA 1; ASNA I; ASNA-I; ASNA_HUMAN; Asna1 protein; ASNAI; ATPase ASNA1; GET3; Golgi to ER traffic 3 homolog; hARSA-I; hASNA-I; MGC3821; Transmembrane domain recognition complex 40 kDa ATPase subunit; Transmembrane domain recognition complex; 40kDa; TRC40; anti-ASNA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ASNA1 Antibody detects endogenous levels of ASNA1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 348

• Applicable Applications for anti-ASNA1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human ASNA1.
• Tissue Specificity: Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endoplasmic Reticulum; Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using ASNA1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-ASNA1 antibody

Description: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling.

Subunit Structure: Homodimer (By similarity). Component of a transmembrane domain recognition complex (TRC) (By similarity). Interacts with SERP1 and SEC61B (By similarity). Interacts with WRB.

Similarity: Belongs to the arsA ATPase family.

NCBI and Uniprot Product Information

• NCBI GI #: 6647417
• NCBI GeneID: 439
• UniProt Accession #: O43681
• Molecular Weight: Observed: 38 kDa; Predicted: 39 kDa
• NCBI Official Full Name: ATPase ASNA1
• NCBI Official Synonym Full Names: guided entry of tail-anchored proteins factor 3, ATPase
• NCBI Official Symbol: GET3
• NCBI Official Synonym Symbols: ARSA1; ASNA1; TRC40; ARSA-I; ASNA-I
• NCBI Protein Information: ATPase ASNA1
• UniProt Protein Name: ATPase ASNA1
• Protein Family: ATPase
• UniProt Gene Name: ASNA1
• UniProt Entry Name: ASNA_HUMAN

NCBI Description

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]

Uniprot Description

ASNA1: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling. Belongs to the arsA ATPase family.

Protein type: Nucleolus; EC 3.6.-.-; EC 3.6.3.16; Hydrolase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: endoplasmic reticulum; cytoplasm; nucleolus; nucleus

Molecular Function: arsenite transmembrane transporter activity; transporter activity; metal ion binding; ATPase activity; ATP binding

Biological Process: protein insertion into ER membrane; transport; metabolic process; inorganic anion transport