Western Blot (WB)
(Western blot analysis of Gab1 in lysates of NIH-3T3 , using Gab1 Antibody.)
Rabbit Gab1 Polyclonal Antibody | anti-Gab1 antibody
Gab1 Antibody
Gene Names
GAB1; DFNB26
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Gab1; Polyclonal Antibody; Gab1 Antibody; Gab 1; GAB1; GAB1_HUMAN; GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; anti-Gab1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Gab1 Antibody detects endogenous levels of Gab1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
694
Applicable Applications for anti-Gab1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA (Peptide): 1:20000-1:40000
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human Gab1.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Gab1 in lysates of NIH-3T3 , using Gab1 Antibody.)
Western Blot (WB)
(Western blot analysis of Gab1 in lysates of NIH-3T3 , using Gab1 Antibody.)
Related Product Information for anti-Gab1 antibody
Description: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR).
Post Translational Modifications: Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Subunit Structure: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK.
Similarity: Belongs to the GAB family.
Post Translational Modifications: Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Subunit Structure: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK.
Similarity: Belongs to the GAB family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 76.6 kDa
Predicted: 77 kDa
Predicted: 77 kDa
NCBI Official Full Name
GRB2-associated-binding protein 1 isoform b
NCBI Official Synonym Full Names
GRB2 associated binding protein 1
NCBI Official Symbol
GAB1
NCBI Official Synonym Symbols
DFNB26
NCBI Protein Information
GRB2-associated-binding protein 1
UniProt Protein Name
GRB2-associated-binding protein 1
Protein Family
UniProt Gene Name
GAB1
UniProt Entry Name
GAB1_HUMAN
NCBI Description
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Research Articles on Gab1
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Product Notes
The Gab1 gab1 (Catalog #AAA9611161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gab1 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Gab1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the Gab1 gab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gab1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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