Rabbit anti-Human Gab 1 Polyclonal Antibody | anti-Gab1 antibody

Gab 1 (Phospho-Y627) Polyclonal Antibody

Cat. Num. AAA3005326

• Gene Names: GAB1; DFNB26
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Gab 1; Polyclonal Antibody; Gab 1 (Phospho-Y627) Polyclonal Antibody; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1; GAB1; anti-Gab1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Gab 1 (Y627) polyclonal antibody detects endogenous levels of Gab 1 protein only when phosphorylated at Tyr627.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 694

• Applicable Applications for anti-Gab1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Gab 1 around the phosphorylation site of Tyrosine 627.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Gab 1  (Y627) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug))

Related Product Information for anti-Gab1 antibody

Gab 1 (GRB2-associated binder 1), one such member of the IRS family, plays an important role in cellular growth response, transformation and apoptosis. Gab 1 is a multi-substrate docking protein that functions downstream in the signaling pathways of different receptor kinases, including EGFR. Gab1 is tyrosine phosphorylated normally in response to insulin and consequently enhances phosphatidylinositol 3- kinase (PI3K) binding. In response to osmotic shock, tyrosine-phosphorylated Gab 1 (p-Gab 1) also binds and activates phosphatidylinositol 3-kinase, suggesting that Gab 1 is the major site for PI3K recruitment following osmotic shock stimulation.

NCBI and Uniprot Product Information

• NCBI GI #: 46370073
• NCBI GeneID: 2549
• NCBI Accession #: NP_002030.2
• NCBI GenBank Nucleotide #: NP_002030.2
• UniProt Accession #: Q13480
• Molecular Weight: ~ 115kDa
• NCBI Official Full Name: GRB2-associated-binding protein 1 isoform b
• NCBI Official Synonym Full Names: GRB2 associated binding protein 1
• NCBI Official Symbol: GAB1
• NCBI Official Synonym Symbols: DFNB26
• NCBI Protein Information: GRB2-associated-binding protein 1
• UniProt Protein Name: GRB2-associated-binding protein 1
• Protein Family: GPI transamidase component
• UniProt Gene Name: GAB1
• UniProt Entry Name: GAB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]