Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using Gab1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 120 kDa.)
Rabbit GAB1 Polyclonal Antibody | anti-GAB1 antibody
GAB1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
GAB1; Polyclonal Antibody; GAB1 Antibody; Gab 1; GAB1_HUMAN; GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; anti-GAB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
GAB1 Antibody detects endogenous levels of total GAB1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GAB1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human GAB1.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue (s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Subunit Structure
Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Part of a tripartite complex containing GAB1, METTL13 and SPRY2. Interacts with METTL13.
Similarity
Belongs to the GAB family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using Gab1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 120 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using Gab1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 120 kDa.)
Related Product Information for anti-GAB1 antibody
Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,616 Da
NCBI Official Full Name
GRB2-associated-binding protein 1 isoform b
NCBI Official Synonym Full Names
GRB2-associated binding protein 1
NCBI Official Symbol
GAB1
NCBI Protein Information
GRB2-associated-binding protein 1; GRB2-associated binder 1; growth factor receptor bound protein 2-associated protein 1
UniProt Protein Name
GRB2-associated-binding protein 1
Protein Family
UniProt Gene Name
GAB1
UniProt Entry Name
GAB1_HUMAN
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Product Notes
The GAB1 gab1 (Catalog #AAA9612556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAB1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the GAB1 gab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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