Western Blot (WB)
(Western blot analysis of eEF2 in lysates of NIH-3T3 , using eEF2 Antibody.)
Rabbit eEF2 Polyclonal Antibody | anti-eEF2 antibody
eEF2 Antibody
Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of eEF2 in lysates of NIH-3T3 , using eEF2 Antibody.)
Western Blot (WB)
(Western blot analysis of eEF2 in lysates of NIH-3T3 , using eEF2 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from PC12, using eEF2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from PC12, using eEF2 Antibody. The lane on the left was treated with blocking peptide.)
Post Translational Modifications: Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis (By similarity). ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. Sumoylated following phosphorylation by CSK, promotes proteolytic cleavage.
Subunit Structure: Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9 (PubMed:19417104). Interacts with RBPMS2 (PubMed:25064856).
Similarity: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
NCBI and Uniprot Product Information
Predicted: 96 kDa
NCBI Description
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
Uniprot Description
EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation.
Protein type: Translation elongation; Translation
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: polysome; membrane; cytoplasm; plasma membrane; nucleus; cytosol; ribonucleoprotein complex
Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity
Biological Process: cellular protein metabolic process; translational elongation; positive regulation of translation; translation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; pathogenesis; gene expression; hemopoietic progenitor cell differentiation; post-translational protein modification
Disease: Spinocerebellar Ataxia 26
Research Articles on eEF2
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Product Notes
The eEF2 eef2 (Catalog #AAA9611009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eEF2 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's eEF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the eEF2 eef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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