Rabbit eEF2 Polyclonal Antibody | anti-eEF2 antibody

eEF2 Antibody

Cat. Num. AAA9611008

• Gene Names: EEF2; EF2; EF-2; EEF-2; SCA26
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: eEF2; Polyclonal Antibody; eEF2 Antibody; EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26; anti-eEF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: eEF2 Antibody detects endogenous levels of eEF2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 858

• Applicable Applications for anti-eEF2 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human eEF2.
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of eEF2 in lysates of NIH-3T3 , using eEF2 Antibody.)

Related Product Information for anti-eEF2 antibody

Description: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Post Translational Modifications: Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis (By similarity). ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. Sumoylated following phosphorylation by CSK, promotes proteolytic cleavage.

Subunit Structure: Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9 (PubMed:19417104). Interacts with RBPMS2 (PubMed:25064856).

Similarity: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 4503483
• NCBI GeneID: 1938
• NCBI Accession #: NP_001952.1
• NCBI GenBank Nucleotide #: NP_001952.1
• UniProt Accession #: P13639
• Molecular Weight: Observed: 95 kDa; Predicted: 96 kDa
• NCBI Official Full Name: elongation factor 2
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 2
• NCBI Official Symbol: EEF2
• NCBI Official Synonym Symbols: EF2; EF-2; EEF-2; SCA26
• NCBI Protein Information: elongation factor 2
• UniProt Protein Name: Elongation factor 2
• Protein Family: Elongation factor
• UniProt Gene Name: EEF2
• UniProt Synonym Gene Names: EF2; EF-2
• UniProt Entry Name: EF2_HUMAN

NCBI Description

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: polysome; membrane; cytoplasm; plasma membrane; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of translation; translation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; pathogenesis; gene expression; hemopoietic progenitor cell differentiation; post-translational protein modification

Disease: Spinocerebellar Ataxia 26