Western Blot (WB)
(Western blot analysis of extracts of Mouse hippocampal tissue sample,using Phospho-Afadin (Tyr1230) Antibody .)
Rabbit Afadin Polyclonal Antibody | anti-AFAD antibody
Phospho-Afadin (Tyr1230) Antibody
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of extracts of Mouse hippocampal tissue sample,using Phospho-Afadin (Tyr1230) Antibody .)
Western Blot (WB)
(Western blot analysis of extracts of Mouse hippocampal tissue sample,using Phospho-Afadin (Tyr1230) Antibody .)
Subunit Structure: Homodimer. Interacts with F-actin, nectin and NECTIN3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.
Similarity: The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminal domain interacts with F-actin.
NCBI and Uniprot Product Information
Predicted: 207 kDa
NCBI Description
This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]
Uniprot Description
Afadin: plays a role in adhesion by participating, along with E-cadherin and catenin, in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Essential for the association of nectin and E-cadherin. Contains 1 forkhead-associated (FHA) domain, a phosphopeptide recognition domain; 1 dilute (DIL) domain, a myosin-like domain which may play a functional role in cellular processes or vectorial vesicle transport; 1 PDZ domain, which frequently binds the carboxyl-terminal sequences of proteins or phosphatidylinositol 4,5-bisphosphate in the plasma membrane; and 2 RA domains, which are associated with RasGTP effector activity. Five alternatively spliced isoforms have been described. Isoform 2 does not interact with F-actin.
Protein type: Oncoprotein; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 6q27
Cellular Component: nucleoplasm; cell-cell adherens junction; apical part of cell; cytoplasm; plasma membrane; intercellular junction; cytosol; cell junction
Molecular Function: protein C-terminus binding; protein binding; Ras GTPase binding; cell adhesion molecule binding
Biological Process: intercellular junction assembly and maintenance; cell-cell signaling; cell adhesion; signal transduction
Research Articles on AFAD
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Product Notes
The AFAD mllt4 (Catalog #AAA9610687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Afadin (Tyr1230) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Afadin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the AFAD mllt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Afadin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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