Rabbit PDK1 Polyclonal Antibody | anti-PDK1 antibody

Phospho-PDK1 (Thr245) Antibody

Cat. Num. AAA9610477

• Gene Names: PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: PDK1; Polyclonal Antibody; Phospho-PDK1 (Thr245) Antibody; 3 phosphoinositide dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 1; hPDK 1; hPDK1; MGC20087; MGC35290; OTTHUMP00000159109; OTTHUMP00000159110; OTTHUMP00000174525; Pdpk1; PDPK1_HUMAN; PDPK2; PDPK2P; PkB kinase; PkB kinase like gene 1; PkB like 1; PRO0461; Protein kinase; anti-PDK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-PDK1 (Thr245) Antibody detects endogenous levels of PDK1 only when phosphorylated at Thr245.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 454

• Applicable Applications for anti-PDK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PDK1 around the phosphorylation site of Thr245.
• Tissue Specificity: Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
• Subcellular Location: Nucleus; Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemmistry (IHC)

(At 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemistry (IHC)

(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Western Blot (WB)

(Western blot analysis of Phospho-PDK1 (Thr245) in lysates of HepG2?, using Phospho-PDK1 (Thr245) Antibody.)

Related Product Information for anti-PDK1 antibody

Description: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

Post Translational Modifications: Phosphorylation on Ser-241 in the activation loop is required for full activity. PDPK1 itself can autophosphorylate Ser-241, leading to its own activation. Autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2 (By similarity). Tyr-9 phosphorylation is critical for stabilization of both PDPK1 and the PDPK1/SRC complex via HSP90-mediated protection of PDPK1 degradation. Angiotensin II stimulates the tyrosine phosphorylation of PDPK1 in vascular smooth muscle in a calcium- and SRC-dependent manner. Phosphorylated on Tyr-9, Tyr-373 and Tyr-376 by INSR in response to insulin. Palmitate negatively regulates autophosphorylation at Ser-241 and palmitate-induced phosphorylation at Ser-529 and Ser-501 by PKC/PRKCQ negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylation at Thr-354 by MELK partially inhibits kinase activity, the inhibition is cooperatively enhanced by phosphorylation at Ser-394 and Ser-398 by MAP3K5. Autophosphorylated; autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2. Monoubiquitinated in the kinase domain, deubiquitinated by USP4.

Subunit Structure: Homodimer in its autoinhibited state. Active as monomer. Interacts with NPRL2, PPARG, PAK1, PTK2B, GRB14, PKN1 (via C-terminus), STRAP and IKKB. The Tyr-9 phosphorylated form interacts with SRC, RASA1 and CRK (via their SH2 domains). Interacts with SGK3 in a phosphorylation-dependent manner. The tyrosine-phosphorylated form interacts with PTPN6. The Ser-241 phosphorylated form interacts with YWHAH and YWHAQ. Binds INSR in response to insulin. Interacts (via PH domain) with SMAD3, SMAD4 and SMAD7. Interacts with PKN2; the interaction stimulates PDPK1 autophosphorylation, its PI(3,4,5)P3-dependent kinase activity toward 'Ser-473' of AKT1 but also activates its kinase activity toward PRKCD and PRKCZ.

Similarity: The PH domain plays a pivotal role in the localization and nuclear import of PDPK1 and is also essential for its homodimerization. The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 387849239
• NCBI GeneID: 5170
• NCBI Accession #: NP_001248745.1
• NCBI GenBank Nucleotide #: NP_001248745.1
• UniProt Accession #: O15530; Q6A1A2
• Molecular Weight: Observed: 63 kDa; Predicted: 64 kDa
• NCBI Official Full Name: 3-phosphoinositide-dependent protein kinase 1 isoform 3
• NCBI Official Synonym Full Names: 3-phosphoinositide dependent protein kinase 1
• NCBI Official Symbol: PDPK1
• NCBI Official Synonym Symbols: PDK1; PDPK2; PDPK2P; PRO0461
• NCBI Protein Information: 3-phosphoinositide-dependent protein kinase 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDPK1
• UniProt Synonym Gene Names: PDK1; hPDK1
• UniProt Entry Name: PDPK1_HUMAN

Uniprot Description

PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKB family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; phospholipase binding; 3-phosphoinositide-dependent protein kinase activity; kinase activity; protein kinase binding; insulin receptor binding; ATP binding; phospholipase activator activity

Biological Process: focal adhesion formation; regulation of mast cell degranulation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; synaptic transmission; regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; activation of protein kinase B; calcium-mediated signaling; peptidyl-threonine phosphorylation; hyperosmotic response; cellular response to insulin stimulus; T cell costimulation; insulin receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transforming growth factor beta receptor signaling pathway; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway