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Typical Testing Data/Standard Curve (for reference only)

Rat Estrogen Related Receptor Alpha (ERRa) ELISA Kit | ERRa elisa kit

Rat Estrogen Related Receptor Alpha (ERRa) ELISA Kit

Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Rat
Synonyms
Estrogen Related Receptor Alpha (ERRa); Rat Estrogen Related Receptor Alpha (ERRa) ELISA Kit; ERR1; ERRa; ERRalpha; ESRL1; NR3B1; Estrogen receptor-like 1; Nuclear receptor subfamily 3 group B member 1; ERRa elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Estrogen Related Receptor Alpha (ERRa).
No significant cross-reactivity or interference between Estrogen Related Receptor Alpha (ERRa) and analogues was observed.
Sequence Length
422
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.063ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Estrogen Related Receptor Alpha (ERRa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Estrogen Related Receptor Alpha (ERRa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ERRa elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Related Receptor Alpha (ERRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Related Receptor Alpha (ERRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Related Receptor Alpha (ERRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Related Receptor Alpha (ERRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,439 Da
NCBI Official Full Name
Estrogen-related receptor alpha
NCBI Official Synonym Full Names
estrogen related receptor alpha
NCBI Official Symbol
ESRRA
NCBI Official Synonym Symbols
ERR1; ERRa; ESRL1; NR3B1; ERRalpha
NCBI Protein Information
steroid hormone receptor ERR1
UniProt Protein Name
Steroid hormone receptor ERR1
UniProt Gene Name
ESRRA
UniProt Synonym Gene Names
ERR1; ESRL1; NR3B1; ERR-alpha

NCBI Description

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.

Research Articles on ERRa

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Product Notes

The Rat ERRa esrra (Catalog #AAA2705319) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2705319 ELISA Kit recognizes Rat ERRa. It is sometimes possible for the material contained within the vial of "Estrogen Related Receptor Alpha (ERRa), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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