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Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Rabbit SLC16A1 Polyclonal Antibody | anti-SLC16A1 antibody
Phospho-SLC16A1 (Ser213) Antibody
Gene Names
SLC16A1; MCT; HHF7; MCT1; MCT1D
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
SLC16A1; Polyclonal Antibody; Phospho-SLC16A1 (Ser213) Antibody; FLJ36745; HHF7; MCT 1; MCT; MGC44475; Monocarboxylate transporter 1; Monocarboxylate transporter; Monocarboxylate transporter isoform 1; Monocarboxylic acid transporter 1; MOT1_HUMAN; Slc16a1; SLC16A1 protein; Solute carrier family 16 (monocarboxylic acid transporters) member 1; Solute carrier family 16 member 1 (monocarboxylic acid transporter 1); Solute carrier family 16 member 1; anti-SLC16A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-SLC16A1(Ser213) Antibody detects endogenous levels of SLC16A1 only when phosphorylated at Ser213.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
500
Applicable Applications for anti-SLC16A1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human SLC16A1 around the phosphorylation site of Ser213.
Tissue Specificity
Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.
Subcellular Location
Plasma Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of extracts of Mouse pancreas? tissue sample,using Phospho-SLC16A1(Ser213) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse pancreas? tissue sample,using Phospho-SLC16A1(Ser213) Antibody.)
Related Product Information for anti-SLC16A1 antibody
Description: Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.
Subunit Structure: Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane (By similarity). Interacts with BSG; this is required for expression at the cell membrane.
Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Subunit Structure: Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane (By similarity). Interacts with BSG; this is required for expression at the cell membrane.
Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 54 kDa
Predicted: 54 kDa
Predicted: 54 kDa
NCBI Official Full Name
monocarboxylate transporter 1
NCBI Official Synonym Full Names
solute carrier family 16 member 1
NCBI Official Symbol
SLC16A1
NCBI Official Synonym Symbols
MCT; HHF7; MCT1; MCT1D
NCBI Protein Information
monocarboxylate transporter 1
UniProt Protein Name
Monocarboxylate transporter 1
Protein Family
UniProt Gene Name
SLC16A1
UniProt Synonym Gene Names
MCT1; MCT 1
UniProt Entry Name
MOT1_HUMAN
NCBI Description
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]
Research Articles on SLC16A1
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Product Notes
The SLC16A1 slc16a1 (Catalog #AAA9610406) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SLC16A1 (Ser213) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC16A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SLC16A1 slc16a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC16A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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