Mouse anti-Zebrafish Tubulin beta Monoclonal Antibody | anti-TUBB antibody

Tubulin beta Antibody

Cat. Num. AAA9610330

• Gene Names: TUBB; M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
• Reactivity: Zebrafish
• Applications: Western Blot, ELISA
• Purity: Affinity Purification
• Synonyms: Tubulin beta; Monoclonal Antibody; Tubulin beta Antibody; TUBB; Beta Tubulin; Beta 5-tubulin; Beta-4 tubulin; Beta1-tubulin; M40; Tubulin beta-5 chain; Beta Ib tubulin; Class I beta-tubulin; OK/SW-cl.56; TUBB5; Tubulin beta chain; Tubulin beta polypeptide; Tubulin beta-1 chain; Tubulin; beta class I; Tubuli...; anti-TUBB antibody

• Host: Mouse
• Reactivity: Zebrafish
• Clonality: Monoclonal
• Specificity: Thebeta-tubulin antibody can detects Zebrafish endogenousbeta-tubulin protein.
• Purity/Purification: Affinity Purification
• Form/Format: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 464

• Applicable Applications for anti-TUBB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:3000-1:10000
• Immunogen: Peptide
• Subcellular Location: Cytoplasm, Cytoskeleton.
• Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
• Preparation and Storage: Store at -20 degree C. Do not aliquot the antibody.

Western Blot (WB)

(Western blot analysis of Tubulin beta Antibody expression in A549 cells lysates.)

Related Product Information for anti-TUBB antibody

Description: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha-and beta-tubulin, each of about 55, 000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit Structure: Heterodimer of alpha and beta chains (PubMed:26637975). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with PIFO (PubMed:20643351). Interacts with DIAPH1 (PubMed:23325789). Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity).
Post-translational Modifications: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Similarity: The highly acidic C-terminal region may bind cations such as calcium. Belongs to the tubulin family.

NCBI and Uniprot Product Information

• NCBI GI #: 645912968
• NCBI GeneID: 203068
• NCBI Accession #: NP_001280141.1
• NCBI GenBank Nucleotide #: NM_001293212.1
• UniProt Accession #: P07437; P05218; Q8WUC1; Q9CY33
• Molecular Weight: Observed: 55 kD; Predicted: 50 kDa
• NCBI Official Full Name: tubulin beta chain isoform a
• NCBI Official Synonym Full Names: tubulin beta class I
• NCBI Official Symbol: TUBB
• NCBI Official Synonym Symbols: M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
• NCBI Protein Information: tubulin beta chain
• UniProt Protein Name: Tubulin beta chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB
• UniProt Synonym Gene Names: TUBB5

NCBI Description

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.