Rabbit anti-Human EAAT1 Polyclonal Antibody | anti-EAAT1 antibody

EAAT1 Antibody

Cat. Num. AAA9608851

• Gene Names: SLC1A3; EA6; EAAT1; GLAST; GLAST1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: EAAT1; Polyclonal Antibody; EAAT1 Antibody; EA6; EAA1_HUMAN; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST-1; GLAST1; Glial high affinity glutamate transporter; glutamate/aspartate transporter; high affinity; sodium-dependent; High affinity neuronal glutamate transporter; Slc1a3; Sodium dependent glutamate/aspartate transporter; Sodium-dependent glutamate/aspartate transporter 1; Solute carrier family 1 (glial high affinity glutamate transporter) member 3; Solute carrier family 1 member 3; anti-EAAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EAAT1 antibody detects endogenous levels of total EAAT1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 497

• Applicable Applications for anti-EAAT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human EAAT1
• Subcellular Location: Membrane.
• Tissue Specificity: Detected in brain (PubMed:8218410, PubMed:7521911, PubMed:8123008). Detected at very much lower levels in heart, lung, placenta and skeletal muscle (PubMed:7521911, PubMed:8123008). Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia (PubMed:7521911).
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (92%), Chicken (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of EAAT1 using COLO205 whole cell lysates)

Related Product Information for anti-EAAT1 antibody

Function: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (PubMed:20477940). Mediates Cl-flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).
Subunit Structure: Homotrimer.
Post-translational Modifications: Glycosylated.
Similarity: Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move. Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A3 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 262359914
• NCBI GeneID: 6507
• NCBI Accession #: NP_001160167.1
• NCBI GenBank Nucleotide #: NM_001166695.2
• UniProt Accession #: P43003; Q4JCQ8; B2R5T3
• Molecular Weight: Observed: 59 kDa; Predicted: 60 kDa
• NCBI Official Full Name: excitatory amino acid transporter 1 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 1 member 3
• NCBI Official Symbol: SLC1A3
• NCBI Official Synonym Symbols: EA6; EAAT1; GLAST; GLAST1
• NCBI Protein Information: excitatory amino acid transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• UniProt Gene Name: SLC1A3
• UniProt Synonym Gene Names: EAAT12 PublicationsManual assertion based on opinion iniRef.4; GLAST-11 PublicationManual assertion based on opinion iniRef.4

NCBI Description

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

Uniprot Description

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (PubMed:20477940). Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate ().