Rabbit EAAT1 Polyclonal Antibody | anti-SLC1A3 antibody

Anti-EAAT1 Antibody

Cat. Num. AAA178459

• Gene Names: SLC1A3; EA6; EAAT1; GLAST; GLAST1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: EAAT1; Polyclonal Antibody; Anti-EAAT1 Antibody; EA6; EAA1; EAAT 1; GLAST; GLAST1; Glast; GLAST-1; SLC1A3; P43003 ; Excitatory amino acid transporter 1; solute carrier family 1 (glial high affinity glutamate transporter); member 3; anti-SLC1A3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

• Applicable Applications for anti-SLC1A3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot (WB); Concentration: 0.1-0.5ug/ml; Tested Species: Mouse, Rat; Predicted Species: Human; Antigen Retrieval: -; ; Immunohistochemistry (IHC) Paraffin; Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Predicted Species: -; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities. ; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. ; ; Other applications have not been tested. Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT1 (14-42aa RMERFQQGVRKRTLLAKKKVQNITKEDVK), different from the related mouse sequence by three amino acids, and identical to the related rat sequence.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of EAAT1 expression in rat brain extract (lane 1) and mouse brain extract (lane 2). EAAT1 at 60KD was detected using rabbit anti-EAAT1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(EAAT1 was detected in paraffin-embedded sections of mouse cerebellum tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(EAAT1 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(EAAT1 was detected in paraffin-embedded sections of huaman glioma tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-SLC1A3 antibody

Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. It is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). And this gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Furthermore, this gene acts as a symport by cotransporting sodium.

References

1. de Vries, B., Mamsa, H., Stam, A. H., Wan, J., Bakker, S. L. M., Vanmolkot, K. R. J., Haan, J., Terwindt, G. M., Boon, E. M. J., Howard, B. D., Frants, R. R., Baloh, R. W., Ferrari, M. D., Jen, J. C., van den Maagdenberg, A. M. J. M. Episodic ataxia associated with EAAT1 mutation C186S affecting glutamate reuptake. Arch. Neurol. 66: 97-101, 2009. Note: Errata: Arch. Neurol. 66: 497 only, 2009; Arch. Neurol. 66: 772 only, 2009.
2. Matsugami, T. R., Tanemura, K., Mieda, M., Nakatomi, R., Yamada, K., Kondo, T., Ogawa, M., Obata, K., Watanabe, M., Hashikawa, T., Tanaka, K. Indispensability of the glutamate transporters GLAST and GLT1 to brain development. Proc. Nat. Acad. Sci. 103: 12161-12166, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 262359914
• NCBI GeneID: 6507
• NCBI Accession #: NP_001160167.1
• NCBI GenBank Nucleotide #: NM_001166695.2
• UniProt Accession #: P43003; Q4JCQ8; B2R5T3
• NCBI Official Full Name: excitatory amino acid transporter 1 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 1 member 3
• NCBI Official Symbol: SLC1A3
• NCBI Official Synonym Symbols: EA6; EAAT1; GLAST; GLAST1
• NCBI Protein Information: excitatory amino acid transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: SLC1A3
• UniProt Synonym Gene Names: EAAT1; GLAST; GLAST1; GLAST-1
• UniProt Entry Name: EAA1_HUMAN

NCBI Description

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

Uniprot Description

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane; plasma membrane

Molecular Function: amino acid transmembrane transporter activity; high-affinity glutamate transmembrane transporter activity; L-glutamate transmembrane transporter activity

Biological Process: glutamate secretion; ion transport; L-glutamate import; neurotransmitter uptake; positive regulation of defense response to virus by host; synaptic transmission

Disease: Episodic Ataxia, Type 6