Rabbit anti-Human CCNA2 Polyclonal Antibody | anti-CCNA2 antibody

CCNA2 Antibody

Cat. Num. AAA9608231

• Gene Names: CCNA2; CCN1; CCNA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CCNA2; Polyclonal Antibody; CCNA2 Antibody; CCN1; CCNA; Ccna2; CCNA2_HUMAN; Cyclin A2; Cyclin-A; Cyclin-A2; anti-CCNA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CCNA2 antibody detects endogenous levels of total CCNA2
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-CCNA2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human CCNA2
• Subcellular Location: Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
• Tissue Specificity: Accumulates steadily during G2 and is abruptly destroyed at mitosis. Not detected during the G1 phase of the cell cycle. It accumulates during the DNA synthesis/S phase and disappears as cells progress into mitosis, between prophase and metaphase (at protein level).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CCNA2 using HepG2 whole lysates.)

Related Product Information for anti-CCNA2 antibody

Function: Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
Subunit Structure: Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes (PubMed:1312467, PubMed:7630397, PubMed:8684460, PubMed:8756328). Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2) (PubMed:1312467). Interacts with CDK2; the interaction increases from G1 to G2 (PubMed:1312467). Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm (PubMed:17698606). Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements (PubMed:8684460).
Post-translational Modifications: Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
Similarity: Belongs to the cyclin family. Cyclin AB subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 4502613
• NCBI GeneID: 890
• NCBI Accession #: NP_001228.1
• NCBI GenBank Nucleotide #: NM_001237.4
• UniProt Accession #: P20248; Q2M3U6; Q4W5P4; Q6LER8; A8K7B6
• Molecular Weight: Observed: 60 kDa; Predicted: 49 kDa
• NCBI Official Full Name: cyclin-A2
• NCBI Official Synonym Full Names: cyclin A2
• NCBI Official Symbol: CCNA2
• NCBI Official Synonym Symbols: CCN1; CCNA
• NCBI Protein Information: cyclin-A2
• UniProt Protein Name: Cyclin-A2
• Protein Family: Cyclin
• UniProt Gene Name: CCNA2
• UniProt Synonym Gene Names: CCN1ImportedManual assertion inferred from database entriesiHGNC:1578; CCNAImportedManual assertion inferred from database entriesiHGNC:1578; Cyclin-A

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]

Uniprot Description

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.