CCNA2 recombinant protein

CCNA2, 1-432aa, Human, His tag, E Coli

Cat. Num. AAA204209

• Gene Names: CCNA2; CCN1; CCNA
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CCNA2; 1-432aa; Human; His tag; E Coli; Cyclin A2; CCN1; CCNA; CCNA2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol 0.15M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLGNSA PGPATREAGS ALLALQQTAL QEDQENINPE KAAPVQQPRT RAALAVLKSG NPRGLAQQQR PKTRRVAPLK DLPVNDEHVT VPPWKANSKQ PAFTIHVDEA EKEAQKKPAE SQKIEREDAL AFNSAISLPG PRKPLVPLDY PMDGSFESPH TMDMSIVLED EKPVSVNEVP DYHEDIHTYL REMEVKCKPK VGYMKKQPDI TNSMRAILVD WLVEVGEEYK LQNETLHLAV NYIDRFLSSM SVLRGKLQLV GTAAMLLASK FEEIYPPEVA EFVYITDDTY TKKQVLRMEH LVLKVLTFDL AAPTVNQFLT QYFLHQQPAN CKVESLAMFL GELSLIDADP YLKYLPSVIA GAAFHLALYT VTGQSWPESL IRKTGYTLES LKPCLMDLHQ TYLKAPQHAQ QSIREKYKNS KYHGVSLLNP PETLNL
• Sequence Length: 432

• Applicable Applications for CCNA2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Ships on dry ice. Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to 80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CCNA2 recombinant protein

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Recombinant human CCNA2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CCNA2 recombinant protein

Cell Cycle

References

Alvarez-Fernandez,M., et al. (2011) J. Biol. Chem. 286 (38), 33029-33036; Kim,D.H., et al. (2011) Cancer 117 (17), 4080-4091

NCBI and Uniprot Product Information

• NCBI GI #: 4502613
• NCBI GeneID: 890
• NCBI Accession #: NP_001228
• NCBI GenBank Nucleotide #: NM_001237.4
• UniProt Accession #: P20248; Q2M3U6; Q4W5P4; Q6LER8; A8K7B6
• Molecular Weight: 51.1 kDa (456aa)
• NCBI Official Full Name: cyclin-A2
• NCBI Official Synonym Full Names: cyclin A2
• NCBI Official Symbol: CCNA2
• NCBI Official Synonym Symbols: CCN1; CCNA
• NCBI Protein Information: cyclin-A2
• UniProt Protein Name: Cyclin-A2
• Protein Family: Cyclin
• UniProt Gene Name: CCNA2
• UniProt Synonym Gene Names: Cyclin-A

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]

Uniprot Description

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.