Western Blot (WB)
(Western blot analysis of extracts of mouse fetal muscletissue lysate, using SKI antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit SKI Polyclonal Antibody | anti-SKI antibody
SKI Antibody
Gene Names
SKI; SGS; SKV
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SKI; Polyclonal Antibody; SKI Antibody; Avian sarcoma viral (v ski) oncogene homolog; C oncogene; C ski; Proto-oncogene c-Ski; Ski oncogene; Ski oncoprotein; SKI_HUMAN; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog; anti-SKI antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SKI antibody detects endogenous levels of total SKI
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
728
Applicable Applications for anti-SKI antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1000
Immunogen
A synthetic peptide of human SKI
Subcellular Location
Nucleus.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse fetal muscletissue lysate, using SKI antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of mouse fetal muscletissue lysate, using SKI antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-SKI antibody
Description: The Ski protein is a nuclear protooncoprotein that is associated with tumors at high cellular concentrations.It has been shown to interfere with normal cellular functioning by both directly impeding expression of certain genes inside the nucleus of the cell and disrupting signaling proteins that activate genes.This protein may play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. May functions in TGF-beta signaling.
Function: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Subunit Structure: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.
Post-translational Modifications: Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.
Similarity: Belongs to the SKI family.
Function: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Subunit Structure: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.
Post-translational Modifications: Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.
Similarity: Belongs to the SKI family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 80 kDa
Predicted: 81 kDa
Predicted: 81 kDa
NCBI Official Full Name
ski oncogene
NCBI Official Synonym Full Names
SKI proto-oncogene
NCBI Official Symbol
SKI
NCBI Official Synonym Symbols
SGS; SKV
NCBI Protein Information
ski oncogene
UniProt Protein Name
Ski oncogene
Protein Family
UniProt Gene Name
SKI
NCBI Description
This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation. [provided by RefSeq, Oct 2009]
Uniprot Description
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Research Articles on SKI
Similar Products
Product Notes
The SKI ski (Catalog #AAA9607742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SKI Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SKI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1000. Researchers should empirically determine the suitability of the SKI ski for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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