Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using ZDHHC15 antibody.)
Rabbit anti-Human ZDHHC15 Polyclonal Antibody | anti-ZDHHC15 antibody
ZDHHC15 Antibody
Gene Names
ZDHHC15; MRX91; DHHC15
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZDHHC15; Polyclonal Antibody; ZDHHC15 Antibody; DHHC-15; Palmitoyltransferase ZDHHC15; UNQ1969/PRO4501; ZDH15_HUMAN; Zdhhc15; Zinc finger DHHC domain-containing protein 15; anti-ZDHHC15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ZDHHC15 antibody detects endogenous levels of total ZDHHC15
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
328
Applicable Applications for anti-ZDHHC15 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Tissue Specificity
Expressed in placenta, liver, lung, kidney, heart and brain.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using ZDHHC15 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using ZDHHC15 antibody.)
Immunofluorescene (IF)
(MBS9605729 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605729 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-ZDHHC15 antibody
Function: Catalyzes palmitoylation of Cys residues on target proteins. Catalyzes palmitoylation of GAP43 and DLG4/PSD95.
Post-translational Modifications: Autopalmitoylated (in vitro).
Similarity: The DHHC domain is required for palmitoyltransferase activity. Belongs to the DHHC palmitoyltransferase family.
Post-translational Modifications: Autopalmitoylated (in vitro).
Similarity: The DHHC domain is required for palmitoyltransferase activity. Belongs to the DHHC palmitoyltransferase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 38 kD
Predicted: 40 kDa
Predicted: 40 kDa
NCBI Official Full Name
palmitoyltransferase ZDHHC15 isoform 2
NCBI Official Synonym Full Names
zinc finger DHHC-type containing 15
NCBI Official Symbol
ZDHHC15
NCBI Official Synonym Symbols
MRX91; DHHC15
NCBI Protein Information
palmitoyltransferase ZDHHC15
UniProt Protein Name
Palmitoyltransferase ZDHHC15
Protein Family
UniProt Gene Name
ZDHHC15
UniProt Synonym Gene Names
DHHC-15
NCBI Description
The protein encoded by this gene belongs to the DHHC palmitoyltransferase family. Mutations in this gene are associated with mental retardatio X-linked type 91 (MRX91). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
Catalyzes palmitoylation of Cys residues on target proteins. Catalyzes palmitoylation of GAP43 and DLG4/PSD95.
Similar Products
Product Notes
The ZDHHC15 zdhhc15 (Catalog #AAA9605729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZDHHC15 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ZDHHC15 zdhhc15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHHC15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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