Rabbit GPR169 Polyclonal Antibody | anti-GPR169 antibody

GPR169 polyclonal antibody

Cat. Num. AAA3014859

• Gene Names: ZDHHC15; MRX91
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR169; Polyclonal Antibody; GPR169 polyclonal antibody; Palmitoyltransferase ZDHHC15; Acyltransferase ZDHHC15; Zinc finger DHHC domain-containing protein 15; DHHC-15; ZDHHC15; UNQ1969; PRO4501; anti-GPR169 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ZDHHC15 polyclonal antibody detects endogenous levels of ZDHHC15 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR169 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human ZDHHC15
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of ZDHHC15 polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: HepG2 whole cell lysateLaneC: Aml-12 whole cell lysateLaneD: C6 whole cell lysate)

Related Product Information for anti-GPR169 antibody

Palmitoyltransferase ZDHHC15, palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates. Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA. Palmitoylates IGF2R and SORT1, promoting their partitioning to an endosomal membrane subdomain where they can interact with the retromer cargo-selective complex. Thereby, regulates retrograde transport from endosomes to the Golgi apparatus of these lysosomal sorting receptors and plays a role in trafficking of lysosomal proteins. In the nervous system, catalyzes the palmitoylation of DLG4/PSD95 and regulates its synaptic clustering and function in synaptogenesis. Could be involved in the differentiation of dopaminergic neurons and the development of the diencephalon. Could also catalyze the palmitoylation of GAP43. Could also palmitoylate DNAJC5 and regulate its localization to the Golgi membrane. Could also palmitoylate FYN as shown in vitro.

NCBI and Uniprot Product Information

• NCBI GI #: 226342941
• NCBI GeneID: 158866
• NCBI Accession #: NP_001139728.1
• NCBI GenBank Nucleotide #: NM_001146256.1
• UniProt Accession #: Q96MV8; Q3SY30; Q6UWH3; B3KVG7
• Molecular Weight: 39,331 Da
• NCBI Official Full Name: palmitoyltransferase ZDHHC15 isoform 2
• NCBI Official Synonym Full Names: zinc finger, DHHC-type containing 15
• NCBI Official Symbol: ZDHHC15
• NCBI Official Synonym Symbols: MRX91
• NCBI Protein Information: palmitoyltransferase ZDHHC15; DHHC-15; zinc finger, DHHC domain containing 15; zinc finger DHHC domain-containing protein 15
• UniProt Protein Name: Palmitoyltransferase ZDHHC15
• UniProt Gene Name: ZDHHC15
• UniProt Synonym Gene Names: DHHC-15
• UniProt Entry Name: ZDH15_HUMAN

NCBI Description

The protein encoded by this gene belongs to the DHHC palmitoyltransferase family. Mutations in this gene are associated with mental retardatio X-linked type 91 (MRX91). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

ZDHHC15: Palmitoyltransferase specific for GAP43 and DLG4/PSD95. Defects in ZDHHC15 are the cause of mental retardation X- linked type 91 (MRX91). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the DHHC palmitoyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, multi-pass; EC 2.3.1.225; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq13.3

Cellular Component: Golgi apparatus; integral to membrane

Molecular Function: zinc ion binding; palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity

Biological Process: establishment of protein localization; protein palmitoylation; synaptic vesicle maturation

Disease: Mental Retardation, X-linked 91