Immunofluorescence (IF)
(MBS9604869 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse RPS6KC1 Polyclonal Antibody | anti-RPS6KC1 antibody
RPS6KC1 Antibody
Gene Names
RPS6KC1; RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RPS6KC1; Polyclonal Antibody; RPS6KC1 Antibody; 52 kDa ribosomal protein S6 kinase; humS6PKh1; KS6C1_HUMAN; Ribosomal protein S6 kinase 52kD polypeptide 1; Ribosomal protein S6 kinase 52kDa polypeptide 1; Ribosomal protein S6 kinase C1; Ribosomal protein S6 kinase delta 1; Ribosomal protein S6 kinase delta-1; Ribosomal S6 kinase like protein with two PSK domains 118 kDa protein; Ribosomal S6 kinase-like protein with two PSK domains 118 kDa protein; RPK 118; RPK118; RSKL1; S6K-delta-1; S6PKh1; SPHK1 binding protein; SPHK1-binding protein; anti-RPS6KC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
RPS6KC1 antibody detects endogenous levels of total RPS6KC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1054
Applicable Applications for anti-RPS6KC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Membrane. Also found in some small dot-like or ring-shaped early endosome structures.
Tissue Specificity
Highly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9604869 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604869 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using RPS6KC1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using RPS6KC1 antibody.)
Related Product Information for anti-RPS6KC1 antibody
Function: May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell (PubMed:12077123). Plays a role in the recruitment of PRDX3 to early endosomes (PubMed:15750338).
Subunit Structure: Interacts with SPHK1 and phosphatidylinositol 3-phosphate (PubMed:12077123). Interacts (via PX domain) with PRDX3 (PubMed:15750338).
Similarity: The PX domain is essential for its localization to the early endosomes.The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.
Subunit Structure: Interacts with SPHK1 and phosphatidylinositol 3-phosphate (PubMed:12077123). Interacts (via PX domain) with PRDX3 (PubMed:15750338).
Similarity: The PX domain is essential for its localization to the early endosomes.The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 117 kD
Predicted: 119 kDa
Predicted: 119 kDa
NCBI Official Full Name
ribosomal protein S6 kinase delta-1 isoform b
NCBI Official Synonym Full Names
ribosomal protein S6 kinase C1
NCBI Official Symbol
RPS6KC1
NCBI Official Synonym Symbols
RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
NCBI Protein Information
ribosomal protein S6 kinase delta-1
UniProt Protein Name
Ribosomal protein S6 kinase delta-1
Protein Family
UniProt Gene Name
RPS6KC1
UniProt Synonym Gene Names
RPK118; S6K-delta-1
NCBI Description
Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017]
Uniprot Description
May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell (PubMed:12077123). Plays a role in the recruitment of PRDX3 to early endosomes (PubMed:15750338).
Research Articles on RPS6KC1
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Product Notes
The RPS6KC1 rps6kc1 (Catalog #AAA9604869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS6KC1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6KC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RPS6KC1 rps6kc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6KC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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