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Western Blot (WB)
(Western blot analysis of extracts from 293, using MYST2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Rabbit MYST2 Polyclonal Antibody | anti-MYST2 antibody
MYST2 Antibody
Gene Names
KAT7; HBO1; HBOA; MYST2; ZC2HC7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MYST2; Polyclonal Antibody; MYST2 Antibody; Hbo 1; HBO1; HBOa; Histone acetyltransferase binding to hORC1; Histone acetyltransferase binding to ORC1; Histone acetyltransferase KAT7; Histone acetyltransferase MYST2; K(lysine) acetyltransferase 7; KAT 7; KAT7; Lysine acetyltransferase 7; MOZ; MOZ YBF2/SAS3 SAS2 and TIP60 protein; YBF2/SAS3; SAS2 and TIP60 protein 2; MYST 2; MYST histone acetyltransferase 2; MYST protein 2; MYST-2; MYST2_HUMAN; SAS 2; TIP60 protein 2; ZC2HC7; anti-MYST2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MYST2 antibody detects endogenous levels of total MYST2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
581
Applicable Applications for anti-MYST2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus > Nucleoplasm.
Tissue Specificity
Ubiquitously expressed, with highest levels in testis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293, using MYST2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using MYST2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Immunofluorescene (IF)
(MBS9604519 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9604519 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using MYST2 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using MYST2 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using MYST2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using MYST2 antibody.)
Related Product Information for anti-MYST2 antibody
Function: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation (By similarity). Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1 (PubMed:18832067). Specifically represses AR-mediated transcription.
Subunit Structure: Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Interacts with MCM2 and ORC1. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone (PubMed:10930412). Interacts with CDT1 (PubMed:18832067). Interacts with MAP2K1 and CUL1 (By similarity).
Post-translational Modifications: Phosphorylation at Ser-57 by PLK1 during mitosis seems important for prereplicative complex formation and DNA replication licensing, and requires prior phosphorylation at Thr-85 and Thr-88 by CDK1 (PubMed:18250300). Phosphorylated by MAP2K1, which accelerates its degradation (By similarity). Autoacetylation at Lys-432 is required for proper function. Ubiquitinated at Lys-338, leading to proteasomal degradation.
Similarity: The C2HC MYST-type zinc finger is required for interaction with MCM2 and ORC1.The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction. Belongs to the MYST (SAS/MOZ) family.
Subunit Structure: Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Interacts with MCM2 and ORC1. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone (PubMed:10930412). Interacts with CDT1 (PubMed:18832067). Interacts with MAP2K1 and CUL1 (By similarity).
Post-translational Modifications: Phosphorylation at Ser-57 by PLK1 during mitosis seems important for prereplicative complex formation and DNA replication licensing, and requires prior phosphorylation at Thr-85 and Thr-88 by CDK1 (PubMed:18250300). Phosphorylated by MAP2K1, which accelerates its degradation (By similarity). Autoacetylation at Lys-432 is required for proper function. Ubiquitinated at Lys-338, leading to proteasomal degradation.
Similarity: The C2HC MYST-type zinc finger is required for interaction with MCM2 and ORC1.The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction. Belongs to the MYST (SAS/MOZ) family.
References
Yang Y, Guan J, Shaikh AS, Liang Y, Sun L, Wang M, Li D, Qiu C, Li X; Journal: Cell Physiol Biochem. Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 75 kD
Predicted: 71 kDa
Predicted: 71 kDa
NCBI Official Full Name
histone acetyltransferase KAT7 isoform 2
NCBI Official Synonym Full Names
lysine acetyltransferase 7
NCBI Official Symbol
KAT7
NCBI Official Synonym Symbols
HBO1; HBOA; MYST2; ZC2HC7
NCBI Protein Information
histone acetyltransferase KAT7
UniProt Protein Name
Histone acetyltransferase KAT7
UniProt Gene Name
KAT7
UniProt Synonym Gene Names
HBO1; HBOa; MYST2; MYST-2
NCBI Description
The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed. [provided by RefSeq, Oct 2016]
Uniprot Description
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation (). Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1 (PubMed:18832067). Specifically represses AR-mediated transcription.
Research Articles on MYST2
Similar Products
Product Notes
The MYST2 kat7 (Catalog #AAA9604519) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYST2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYST2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MYST2 kat7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYST2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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